齊一生物大鼠腦源性神經(jīng)營(yíng)養(yǎng)因子（BDNF）檢測(cè)試劑盒齊一生物科技（上海）有限公司以真誠(chéng)、主動(dòng)、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評(píng)。公司秉承“適應(yīng)多樣化的需求，提供專業(yè)化的服務(wù)”的經(jīng)營(yíng)理念，為客戶提供*的服務(wù)。歡迎社會(huì)各界朋友前來(lái)洽談業(yè)務(wù)，共創(chuàng)輝煌
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
齊一生物大鼠腦源性神經(jīng)營(yíng)養(yǎng)因子（BDNF）檢測(cè)試劑盒甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
9022022 Sensor, Z-axis, with cable, QXA
9021653 Kit, stickers, eluate drawer, Qsym
9021623 Gateway 2, CC-Link, DCU
9021289 Yellow grease for PM, EZ1
9240671 Reservoir, 500ml w/cap, AP96/196
9240640 Opto Sensor, Interlocks/Latch Lock, AP
9240639 Opto Sensor, Common, AP
9240633 Power Supply, International, AP97
9240614 Fuse, 0.25A, FNM, AP96
9240613 Fuse, 15.0A, FNM, AP96
9240585 Filter Assembly, Pressure, AP196/98
9240580 Cable, Vacuum Airflow Sensor, AP196/98
9232772 Syringe, 2.5ml
9232770 Syringe, 500 μl
9239346 Spotlight bulb (2), BR M48
9239334 Silicone Grease, BR M48 & BR EZ1
9236466 BR 8000, Full Agreement The Full Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9236435 Power entry replacement kit, TWI
9239139 Spacer, support & pod foot, TWII
9239104 Washer set, valve Port S 5.3mm, Kloehn
9239102 Washer set, valve port A/B 4.5mm, Kloehn
9232649 Cooling block, 1-tube 13 ml Holder for accommodating a single tube (13 ml) on the worktable
9232640 Box, used tips
9232635 Bottle, 125-ml, orange fitting
9232633 Bottle, 125-ml, white luer adapter
9238990 Z-Axis Coupling Unit, EZ1
9238924 Bearing, BS15
9238923 Hinge support, BS15
9238784 Swing-out rotor for 6 buckets
9238909 PCB, UI-01 w. LCD/Keypad, BS96
9238740 SBS Vacuum Manifold Top Station
9238713 Eprom 4-15, Zent1, opt. Imbalance
9232329 Cable, LSB to X-main board, BR8000
9232321 Cable set, PS5 to XMAIN, BR8000
9232276 Motor, Z, BR8000
9238698 Eprom 4K15, Zent1, imbalance sensor
9231596 Fitting, 3/2,
9231131 Flat Band Cable 10P Xadp - Yzadp
9231130 Cable, X-adapter to X-motor, power, BR80
9014778 Pump, vacuum, auto vac, UK, BR8000
9020078 Keypad, TLyser 2
9020075 V-Belt, Motor, TLyser 2
9020072 Foot Cover (1), TLyser 2
9020068 Hall Sensor Board, TLyser
9015015 Probe Guide, BR8 RD v1.0
9014852 Rotor unit, BR8000
9014784 Laboratory Cabinet MDx
9014713 Cable, Rotor Door Sensor, BR8 RD
9022198 PyroMark Q96 ID App Software 2.5
9021403 RGQ MDx User manuals for IVD use (US)
9019885 Cover, main, label Biocontrol, RG3
9019745 EZ1 Adv. XL DNA Dried Blood Card
9019729 Board, motor control, Z, V2, QX
9013842 Tip Adapter Kit, 1100ul, BR8000
9013550 Set, Fuses, (220 + 110V) Plato 1/3
9013429 Maintenance kit, semi annual, BR9600 (C)
9013425 Maintenance Kit BR 9604 "See Components tab for what comes in this kit.
Also includes 4x9013641, BGR Valve 4-port for P1/3 Syringe Pump; 1.5m of 9233214 (listed in Components as qty of 1)."
9019668 PCB - Valve, PyroQ24
9019649 Fuse kit (6.3Ax10, 5Ax5), PyroQ96
9019602 Home flag, Y-axis, Cx8
9019599 Housing, filter seal, CX1
9019585 Slideout electrical tray, QGY
9015283 Cover, Z, M48
9015274 Laboratory Cabinet BR8000RD
9001909 QIAgility HEPA/UV (w/o PC) Priority QIAgility HEPA/UV (w/o PC) Priority includes the QIAgility instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor and travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
133118 NeXtalStock tert-Butanol (200)
133109 NeXtalStock Sodium chloride (200)
965652 QIAamp Virus BioRobot MDx Kit (12) For 12 x 96 preps: 12 QIAamp 96 Plates,RNase-free Buffers,† QIAGEN Protease,Elution Microtubes CL, Caps, S-Blocks, Carrier RNA
965162 QIAamp DNA Blood BioRobot 9604 Kit (12) For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
962341 DirectPrep 96 BioRobot Kit (4) For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Reagents, Buffers, Flat-Bottom Blocks and Lids, 96-Well Microplates FB, AirPore Tape Sheets, Tape Pads
9018535 Tool, door sensor deactivation, QIAC
133103 NeXtalStock Propylene glycol (200)
9015513 PM Kit Biorobot M 48
9015470 Motor, Z
9001887 QIAcube (230V), PrioPLUS "QIAcube (230V) Instrument
Installation of QIAcube instrument & Introductory training
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001886 QIAcube (230V), Priority QIAcube (230V), Priority includes the QIAcube instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor and travel costs with a service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
9018459 Safety valve, assy, QX
9018456 Linear guide, Y axis, QX
9012421 MP-Slot Extention
9012397 Holder, vacuum trap, auto vac, BR8000
204854 QuantiFast Multiplex RT-PCR Kit (400)
133174 NeXtalStock CHES pH 10.0 (200)
133173 NeXtalStock CHES pH 9.0 (200)
291043 mericon Screen nos Kit (24)
290135 mericon Vibrio triple Kit (96)
290115 mericon Y. enterocolitica Kit (96)
9015402 O-rings, plunger, M96 (8)
9001731 QIAgility HEPA / UV Instrument
133088 NeXtalStock PEG 550 MME (200)
38025 MAL Cartridge (5ml)
292015 mericon Pig Kit (96)
133123 NeXtalStock Tris (200)
965672 QIAamp One-For-All Nucleic Acid Kit (12)
133192 NeXtalStock Ammonium tartrate pH 7 (200)
133183 NeXtalStock Citric acid pH 3.5 (200)
9018513 Adapter, USB-serial, EZ1a
9001896 QIAxtractor, UV/HEPA (w/o PC) System QIAxtractor, UV/HEPA (w/o PC) System combines the QIAxtractor system and installation. The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation and user maintenance. All travel and labor cost included for Installation. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
51104 QIAamp DNA Blood Mini Kit (50) For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
133099 NeXtalStock Potassium nitrate (200)
133092 NeXtalStock PEG 8,000 (200)
9015516 Heat unit, complete
9018452 Piston, purge (1), QX
9012416 Microplate Station 8000, short Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the BioRobot 8000 or MDx worktable
133168 NeXtalStock CAPSO pH 9.2 (200)
9018424 Door, N2, QX
9015405 Board, PDB, M96
