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Anti PIK3CA(E545K) Rabbit Polyclonal Antibody

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Anti PIK3CA(E545K) Rabbit Polyclonal Antibody

Anti PIK3CA(E545K) Rabbit Polyclonal Antibody 產(chǎn)品信息

PIK3CA(E545K) [21145]
[100 µL]

Catalog Number: 21145

Gene Symbol: PIK3CA(E545K)

Description: Anti PIK3CA(E545K) Rabbit Polyclonal Antibody

Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers.

Immunogen: A synthetic peptide from the internal region of PIK3CA(E545K) of human origin.

Applications: ELISA, WB, IHC, IP

Recommended Dilutions:
ELISA: 1:100-1:5000
WB: 1:100-1:1000
IHC: 1:10-1:100

Concentration: 0.2 mg/ml

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Purity: Purified from serum

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with Anti PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145).

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