Sephadex LH-20
Matrix | Hydroxypropylated, cross-linked dextran |
Partical Size Dry | 18 μm-111 μm |
Particle Size in Methanol Min. | 27μm |
Particle Size in Methanol Max. | 163μm |
Sample Loading Volume | Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode] |
pH Stability Working Range | 2-13 |
pH Stability Cleaning-in-Place (CIP) | 2-13 |
Pressure/Flow Specification | 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d. |
Flow Velocity | < 60 cm/hour |
Fractionation [Mr] Globular Proteins | 4 × 103 (Exclusion limit will depend on the solvent) – 5 × 103 (Exclusion limit will depend on the solvent) |
Exclusion Limit Peptides | 4 x 103 |
Exclusion Limit Small Organics | 5 x 103 |
Storage Conditions | 4 to 30°C |
Estimated Shelf Life from Manufacture Date | 8 years |
Chemical Stability | Stable in most aqueous and organic eluent systems. |
Avoid Using | Not stable below pH 2 nor to strong oxidising agents |
Autoclavable | 20 min at 121°C |