R&D新增加了一些白介素17（IL-17）家族的配體和受體的熒光染料結(jié)合的抗體。IL-17家族配體是誘導(dǎo)局部細(xì)胞因子產(chǎn)生的促炎癥細(xì)胞因子，并參與了免疫功能的調(diào)節(jié)，主要是變態(tài)反應(yīng)。6個(gè)成員，IL-17、IL-17B、IL-17C、IL-17D、IL-17E（也就是IL-25）和IL-17F是結(jié)構(gòu)相關(guān)的蛋白，在C端附近有著保守的半*結(jié)折疊，N端的序列差異相當(dāng)大。IL-17家族成員激活三個(gè)受體——IL-17 R、IL-17B R和IL-17 RC。兩個(gè)與IL-17 R同源的孤兒受體分別命名為IL-17 RD和IL-17 RE，它們的功能配體目前還不清楚。
下面顯示了用IL-17B（圖1）、IL-17C（圖2）或IL-17E（圖3）的新抗體通過(guò)流式細(xì)胞儀檢測(cè)細(xì)胞內(nèi)染色。IL-17B在脊髓高表達(dá)，并結(jié)合IL-17B R，而不結(jié)合IL-17 R。研究表明IL-17B和C刺激人單核細(xì)胞系THP-1中TNF-α和IL-1β的釋放。IL-17E結(jié)合并激活I(lǐng)L-17B R，它在炎癥環(huán)境中上調(diào)。
表1列出了R&D提供的IL-17家族配體和受體的所有熒光染料結(jié)合抗體。另外，R&D還提供了Human IL-17 Fluorokine® Flow Cytometry Kit（貨號(hào)：NF1700），用于檢測(cè)IL-17的功能受體和估計(jì)其在細(xì)胞表面的密度。
Figure 1: Intracellular staining of K562 cells with PE-conjugated anti-human IL-17B monoclonal antibody （Catalog # IC1248P; filled histogram） or with mouse IgG2B isotype control antibody （Catalog # IC0041P; open histogram）.
Figure 2: Intracellular staining of PC-3 cells with PE-conjugated anti-human IL-17C monoclonal antibody （Catalog # IC1234P; filled histogram） or mouse IgG2B isotype control antibody （Catalog # IC003P open histogram）.
Figure 3: Intracellular staining of PC-3 cells with APC-conjugated anti-human IL-17E monoclonal antibody （Catalog # IC1258A; filled histogram） or with mouse IgG2B isotype control antibody （Catalog # IC002A; open histogram）.
| APC=Allophycocyanin PE=Phycoerythrin |
| Molecule | Species | Catalog Number | Type | Label | Size |
| IL-17 | Human | IC3171A | Mouse IgG1 | APC | 100 Tests |
| Human | IC3171P | Mouse IgG1 | PE | 100 Tests |
| IL-17 R | Human | FAB177A | Mouse IgG1 | APC | 100 Tests |
| Human | FAB177F | Mouse IgG1 | Fluorescein | 100 Tests |
| Human | FAB177P | Mouse IgG1 | PE | 100 Tests |
| Mouse | FAB448F NEW | Goat IgG | Fluorescein | 100 Tests |
| IL-17B | Human | IC1248A NEW | Mouse IgG2B | APC | 100 Tests |
| Human | IC1248F NEW | Mouse IgG2B | Fluorescein | 100 Tests |
| Human | IC1248P NEW | Mouse IgG2B | PE | 100 Tests |
| IL-17B R | Human | FAB1207P | Mouse IgG2B | PE | 100 Tests |
| Mouse | FAB1040A NEW | Rat IgG2B | APC | 100 Tests |
| Mouse | FAB1040F NEW | Rat IgG2B | Fluorescein | 100 Tests |
| IL-17C | Human | IC1234A NEW | Mouse IgG2A | APC | 100 Tests |
| Human | IC1234F NEW | Mouse IgG2A | Fluorescein | 100 Tests |
| Human | IC1234P NEW | Mouse IgG2A | PE | 100 Tests |
| IL-17D | Mouse | IC2274A NEW | Rat IgG2A | APC | 100 Tests |
| IL-17E | Human | IC1258A NEW | Mouse IgG1 | APC | 100 Tests |
| Human | IC1258F NEW | Mouse IgG1 | Fluorescein | 100 Tests |
| Human | IC1258P NEW | Mouse IgG1 | PE | 100 Tests |
| IL-17 RC | Mouse | FAB2270F | Goat IgG | Fluorescein | 100 Tests |
| IL-17 RD | Human | FAB2275F | Goat IgG | Fluorescein | 100 Tests |
| Human | FAB2275P | Goat IgG | PE | 100 Tests |
會(huì)員1.png)