Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background:
NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H（+） currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease （X-CGD）. X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.
pecificity :
–anti- NOX2/gp91phox is a rabbit monoclonal antibody, unconjugated
–specific for NOX2/gp91phox of human,Rat and mouse
–use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein A affinity chromatography purification, purity :>95%
–Isotype: IgG
–mol wt: 63kDa
Anti-NOX2/gp91phox
Application :
–Western blotting 1:100-500
–Immunohistochemistry 1:100-500
–ELISA 1:500-1000
–Optimal working dilutions must be determined by the end user.
Storage:
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
