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上海興悠生物科技有限公司
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上海興悠生物科技有限公司
閱讀：198發(fā)布時間：2015-11-04
上海興悠生物科技有限公司
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Rabbit Anti -Skp2 antibody
Catalog Number :XY-1096R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Substrate recognition component of a SCF（SKP1-CUL1-F-box protein） E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1L, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. [PATHWAY] Protein modification; protein ubiquitination. [SUBUNIT] Part of the SCF（SKP2） complex consisting of CUL1, RBX1, SKP1 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin A-CDK2 complex. Interacts with ORC1L, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL. [SIMILARITY] Contains 1 F-box domain. [SIMILARITY] Contains 8 LRR （leucine-rich） repeats.
Specificity :
– Anti - Skp2 is a rabbit polyclonal antibody unconjugated
– specific for Skp2 of hu, rat ，mo
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 47kDa
Anti-Skp2細(xì)胞S相激酶相關(guān)蛋白抗體說明書
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– IF=1:100-500 IP=1:20-100
– ELISA 1:500-1000
– IHC-P=1:100-500 IHC-F=1:100-500
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
商鋪：http://www.kytsldc.cn/st122143/
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