*雙水解酶試驗(yàn)培養(yǎng)基
英文名稱:Double-arginine Hydrolase Test Medium
*雙水解酶試驗(yàn)培養(yǎng)基 相關(guān)產(chǎn)品:
BDS培養(yǎng)基 Bacteroid Selective Agar 250g/瓶 用于擬桿菌的選擇性分離
AN厭氧肉湯基礎(chǔ) Anaerobic Broth Base 250g/瓶 厭氧基礎(chǔ)培養(yǎng)基,每100ml添加S5605和S5606各1支
AN厭氧瓊脂基礎(chǔ) Anaerobic Agar Base 250g/瓶 厭氧基礎(chǔ)培養(yǎng)基,每100ml添加S5605和S5606各1支
葡萄糖血瓊脂基礎(chǔ)(*葡萄糖血瓊脂基礎(chǔ)) Glucose Blood Agar 250g/瓶 用于細(xì)菌的培養(yǎng),需添加5-10%脫纖維綿羊血。每100ml添加1支S4105制備*葡萄糖血瓊脂
厭氧基礎(chǔ)瓊脂 Anaerobe Basal Agar 250g/瓶 用于厭氧菌尤其是擬桿菌的培養(yǎng),需添加5-10%無(wú)菌脫纖維綿羊全血
45. Pea Agar (豌豆瓊脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (瓊脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2計(jì))] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml
of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall
off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter
again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (稱取白豌豆900克,置于5公升廣口瓶中,加自
來水3 公升,用硫酸調(diào)pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37℃恒溫室7-10天。取出用濾
紙過濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120℃30分鐘滅菌。后冷藏備用。)
The standard quality of pea infusion as following (豌豆浸汁質(zhì)量標(biāo)準(zhǔn)):
Aminonitrogen(氨基酸): 160-200 mg∕100ml
Soluble phosphoruse(溶磷): 400-600 μg∕ml
Color(色澤): auratus,transparent (金黃、透明)
46. Wheat Bran Medium (麥麩瓊脂)
Wheat bran (麥麩) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (瓊脂) 2% pH 7.0
適用范圍:*鏈霉菌、*鏈霉菌
47. Glucose Yeast Extract Agar (葡萄糖、酵母膏瓊脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (瓊脂) 15g
pH 7.2
48. MMN Medium (MMN培養(yǎng)基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (檸檬酸) 0.2g
Distilled water (蒸餾水) 900ml pH 5.5 (左右)
適用范圍:蜜環(huán)菌
49. Starch Agar (淀粉瓊脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1000ml
50. 5 Be′Wort Agar (5 Be′麥芽汁瓊脂)
厭氧基礎(chǔ)肉湯 Anaerobe Basal Broth 250g/瓶 用于厭氧菌尤其是擬桿菌的培養(yǎng)
碎肉葡萄糖肉湯基礎(chǔ) Chopped Meat Glucose(CMG) 250g/瓶 用于厭氧菌特別是專性厭氧菌的培養(yǎng),含VK ,需添加S5604
碎肉肉湯基礎(chǔ) Chopped Meat Broths(CM) 250g/瓶 用于厭氧菌特別是專性厭氧菌的培養(yǎng),含VK ,需添加S5604
硫乙醇酸鹽培養(yǎng)基(Brewer) Thioglycollate Medium (Brewer) 250g/瓶 用于厭氧菌培養(yǎng)
TYA培養(yǎng)基基礎(chǔ) TYA Medium Base 250g/瓶 用于厭氧菌的培養(yǎng),每升培養(yǎng)基中需添加3g醋酸銨
改良FM瓊脂 FM Agar, Modified 250g/瓶 用于梭桿菌的分離和培養(yǎng)
庖肉培養(yǎng)基基礎(chǔ) Cooked Meat Medium Base 250g/瓶 用于梭菌或其它厭氧菌的培養(yǎng),需添加適量S5604
梭狀芽孢桿菌計(jì)數(shù)瓊脂 Clostridia Count Agar 250g/瓶 用于厭氧亞硫酸鹽梭狀芽孢桿菌計(jì)數(shù)
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