ESF 158小鼠胚胎干細(xì)胞ESF 158(干細(xì)胞庫(kù)保藏)
小鼠*性胚胎干細(xì)胞,每支細(xì)胞量1×106,來(lái)源于FVB/NJ (FVB)和C57BL/6-Tyrc-2J (c2J)的嵌合鼠,嵌合鼠毛色高表達(dá)c2J,低表達(dá)FVB
小鼠,F(xiàn)VB/NJ (FVB)和C57BL/6-Tyrc-2J (c2J)的嵌合鼠
小鼠胚胎內(nèi)細(xì)胞團(tuán)
球形克隆
mES*培養(yǎng)液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(終濃度為1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 氣相:空氣,95%;二氧化碳,5%。溫度:37攝氏度。
A。僅供研究之用。
Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514 Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137