近日,植物學(xué)刊物《Journal of Experimental Botany》上在線發(fā)表了浙江大學(xué)農(nóng)學(xué)院甘銀波教授研究組題為“Multiple phytohormones promote root hair elongation by regulating a similar set of genes in the root epidermis in Arabidopsis.”的研究論文，本科生張汕為論文的*作者，甘銀波教授為通訊作者。
研究論文系統(tǒng)研究了植物激素包括生長素、乙烯和細(xì)胞分裂素協(xié)同調(diào)控擬南芥根毛形成的分子作用機理，提出了生長素、乙烯、細(xì)胞分裂素和RHD6通過調(diào)節(jié)相同的根毛特異性基因RSL4, EXP7，EXP18，RSH4， RHS10, RHS16促進根毛形成的分子作用模型，進一步補充和完善了植物激素通過鋅指蛋白控制根毛和表皮毛細(xì)胞形成的作用機制，對于如何培育具有較高根系生理活性，特別是具有較多和較長根毛，提高植物根系吸收養(yǎng)分和水分的效率，提高作物產(chǎn)量，具有重要的理論意義和實際應(yīng)用價值。
原文摘要：
Multiple phytohormones, including auxin, ethylene, and cytokinin, play vital roles in regulating cell development in the root epidermis. However, their interactions in specific root hair cell developmental stages are largely unexplored. To bridge this gap, we employed geneticand pharmacological approaches as well as transcriptional analysis inorder to dissect their distinct and overlapping roles in root hairinitiation and elongation in Arabidopsis thaliana. Our results show that among auxin, ethylene, and cytokinin, only ethylene induces ectopicroot hair cells in wild-type plants, implying a special role of ethylene inthe hair initiation stage. In the subsequent elongation stage, however,auxin, ethylene, and cytokinin enhance root hair tip growth equally. Our data also suggest that the effect of cytokinin is independent fromauxin and ethylene in this process. Exogenous cytokinin restores roothair elongation when the auxin and ethylene signal is defective, whereas auxin and ethylene also sustain elongation in the absence ofthe cytokinin signal. Notably, transcriptional analyses demonstrated thatauxin, ethylene, and cytokinin regulate a similar set of root hair-specific genes. Together these analyses provide important clues regarding the mechanism of hormonal interactions and regulation inthe formation of single-cell structures.
