| Synonyms | 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II. |
| Introduction | Matrix metalloproteinase-2 (MMP-2) is a type IV collagenase, which is involved in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. MMP-2 contains a number of distinct domains: a prodomain that is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin like domain believed to have a role in substrate targeting; and a carboxyl terminal (hemopexin like) domain containing 2 N-linked glycosylation. The MMP-2 can degrade an extensive array of substrates including type IV, V, VII and X collagens as well as gelatin type I. In addition, MMP-2 interacts with THBS2, TIMP2, Thrombospondin 1, CCL7 and TIMP4. MMP-2 autocatalytic cleavage in the C-terminal generates the anti-angiogenic peptide, PEX. This process seems to be made possible by binding integrinv/beta3. Defects in the MMP-2 are the cause of Torg-Winchester syndrome (TWS), aka multicentric osteolysis nodulosis and arthropathy (MONA). |
| Description | MMP-2 Human Recombinant produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a ployhistide tag at the C-terminus, having an Mw of 71kDa.
MMP-2 is purified by proprietary chromatographic techniques. |
| Source | HEK293 cells. |
| Physical Appearance | The MMP-2 is supplied as a sterile Filtered colorless solution. |
| Formulation | The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4. |
| Stability | Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Biological Activity | The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001)., The specific activity is > 1,000 pmoles/min/µg.
Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).
Activation Protocol:
1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.
3. Incubate at 37°C for 1 hour. |
| References | Title:MMP-2 regulates human plaet activation by interacting with
integrin aIIbb 3.
Publication:To cite this article: Choi W-S, Jeon O-H, Kim H-H, Kim D-S. MMP-2 regulates human plaet activation by interacting with integrin aIIbb3.
J Thromb Haemost 2008; 6: 517–23.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2007.02871.x/pdf |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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