Free Radical Biology and Medicine doi:10.1016/j.freeradbiomed.2010.01.036
Frog skins keep redox homeostasis by antioxidant peptides with rapid radical scavenging ability Cunbao Liua, b, 1, Jing Hongb, c, 1, Hailong Yanga, b, 1, Jing Wua, b, Dongying Maa, b, Dongsheng Lia, Donghai Linc, , and Ren Laia, d
a Biotoxin Unit of the Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China b Graduate School, Chinese Academy of Sciences, Beijing, China c Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China d Key Laboratory of Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Life Sciences College, Nanjing Agricultural University, Nanjing, Jiangsu, China
The question of how amphibians can protect themselves from reactive oxygen species when exposed to the sun in an oxygen-rich atmosphere is important and interesting, not only from an evolutionary viewpoint, but also as a primer for researchers interested in mammalian skin biology, in which such peptide systems for antioxidant defense are not well studied. The identification of an antioxidant peptide named antioxidin-RL from frog （Odorrana livida） skin in this report supports the idea that a peptide antioxidant system may be a widespread antioxidant strategy among amphibian skins. Its ability to eliminate most of the 2,2′-azino-bis（3-ethylbenzthiazoline-6-sulfonic acid） radical tested within 2 s, which is much faster than the commercial antioxidant factor butylated hydroxytoluene, suggests that it has a potentially large impact on redox homeostasis in amphibian skins. Cys10 is proven to be responsible for its rapid radical scavenging function and tyrosines take part in the binding of antioxidin-RL to radicals according to our nuclear magnetic resonance assay.
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