Rabbit Anti-OSX   （Osterix）抗體說(shuō)明書(shū)

Quantity size :   0.2ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）  

Background:    OSTERIX, or SP7, is a zinc finger-containing, osteoblast-specific transcription factor that is distinctly expressed in all developing bones, and is a key requirement for the differentiation of pre-osteoblasts into fully functional osteoblasts. While research on the actual functional mechanisms of OSTERIX is still on-going, current findings indicate that OSTERIX expression is induced by BMP-2 and Dlx5, and operates downstream of RUNX2, supporting its role in  mesenchymal stem cell differentiation. Further studies also indicate that the OSTERIX transcription factor may also play a role in negatively regulating the Wnt/β-catenin signaling pathways and the proliferation of osteroblast cell types during embryonic osteogenesis. LOCATION: Nucleus （By similarity）.   SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Specificity :       ·anti- Osterix is a rabbit polyclonal antibody unconjugated

· specific for Osterix of human, mouse, rat.  

 ·epitope mapping near the amino terminus of Osterix of human,

mouse, rat, pig.
  · use for western blotting, elisa, immunoprecipitation  

       and immunohistochemistry

·  Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 45kDa

Application:      ·Western blotting 1:100-500

                 ·Immunohistochemistry 1:100-500

                 · ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage:     Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

  Rabbit Anti-OSX   （Osterix）抗體說(shuō)明書(shū)

  Rabbit Anti-OSX   （Osterix）抗體說(shuō)明書(shū)