Rabbit Anti-BDNF抗體說明書

Catalog Number :RS-0248R

Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )

Background:

Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family.

Specificity : Anti-BDNF is a rabbit monoclonal antibody

specific for BDNF of human,rabbit, mouse, rat，cow

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt: 12.5 kDa

Application :

• WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 lmmunohistochem1：100-500
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.