Anti-PR抗體說(shuō)明書(shū)
Background: The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor , PR-A and PR-B, which are transcribed from distinct, estrogen-inducible promoters within a single copy of the PR geneThe first 164 amino acids of PR-B are absent in PR-A. Progesteronebound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity. An inhibitory domain （ID）, which maps to the amino terminus of the receptor , exists within both PR isoforms. Interestingly, the ID is functionally active only in PR-A and is necessary for steroid hormone transrepression by PR-A, suggesting that PR-A and PR-B may have different conformations in the cell. Phosphorylation of human PR occurs on at least nine serine residues. Phosphorylation of three of the residues is hormoneinducible （Ser 102, Ser 294 and Ser 345）; the others are basal but hormonestimulated.
pecificity :
– Anti-PR is a rabbit polyclonal antibody unconjugated
– specific for PR of human, mouse, rat and pig
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 121kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-PR抗體說(shuō)明書(shū)
