Anti-BrdU （Bromodeoxyuridine） 抗體說(shuō)明書(shū)
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background :
Bromodeoxyuridine （BrdU） is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood （Campana et al., 1988）, tissues （Schutte et al., 1987; Hayashi, et al., 1988）, tumors （Hoshino et al., 1986; Morstyn et al., 1983）, as well as for determining plasma cell labeling indices （Greipp et al., 1985）.
Anti-BrdU （Bromodeoxyuridine） 抗體說(shuō)明書(shū)
Specificity :
· anti- BrdU is a rabbit monoclonal antibody unconjugated
·· specific for BrdU of human,Canine, rat,mouse and chicken
· use for elisa, immunoprecipitation and immunohistochemistry
· affinity chromatography purification, purity :>95%
· Isotype: IgG
Application:
· Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage:
Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-BrdU （Bromodeoxyuridine） 抗體說(shuō)明書(shū)
