Anti-C-Met/Met/HGFR

Catalog Number : Rs-0668R

Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBSor antibody diluent )

Background:

c-Met, a member of the tyrosine kinase superfamily, isthe receptor for hepatocyte growth factor, also known as scatter factor(HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer withMr=190 kDa composed of a heavily glycosylated alpha subunit that is compleyextracellular in localization, and a beta subunit comprising an extracellularligand binding domain, a single transmembrane domain, and a cytoplasmictyrosine kinase domain. Cells expressing c-Met include epithelial cells,endothelial cells, blood cells of various types, and glomerular mesenchymalcells.HGF/SF binding to c-Met stimulates receptor dimerization and thephosphorylation of numerous residues within the receptor’s cytoplasmic domain.Signaling proteins that are phosphorylated and/or localized in response toc-Met phosphorylation include: Grb2, Shc, Cbl, Crk, cortactin, paxillin, GAB1,PI3K, FAK, Src, Ras, ERK1 and 2, JNK, PLC gamma, AKT, and STAT3. HGF/SFstimulation of c-Met expressing cells enhances proliferation, migration,morphogenesis, and protease synthesis, characteristics that are associated withinvasive cell phenotype. Many types

of cancer exhibit sustained c-Met stimulation,overexpression, or mutation, including carcinomas of the colon, breast, ovary,lung, liver, prostate, thyroid, kidney, as well as melanomas and sarcomas. Inaddition to cancer studies, other research areas in which c-Met is underinvestigation include organogenesis, organ regeneration, angiogenesis andsurgical wound healing.

pecificity :    

-  Anti-C-Met isa mouse  monoclonal  antibody unconjugated                

specific for Met of  Human, Rat

–    use for westernblotting,elisa,immunoprecipitation and immunohistochemistry

–    Protein G affinity chromatographypurification, purity :>95%

–    Isotype:  IgG

–    mol wt:153kDa

Application :  

–     WB=1:100-500 ELISA=1:500-1000 IP=1:20-100  IHC-P=1:100-500 IHC-F=1:100-500  IF=1:100-500
not yet tested in other applications.

–     optimaldilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thawcycles. The  lyophilized antibody isstable at room temperature for at least one month and for greater  than a year when kept at -20℃. Whenreconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for atleast six weeks at 2-4 ℃

Important Note: Thisproduct as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.