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產(chǎn)品介紹 | |||
| 品名 | Rabbit anti-Calbindin D-28k | ||
| 貨號(hào) | CB-38a (new batch) | ||
| 規(guī)格 | 200 µl | ||
| 克隆性 | 多克隆 | ||
| 共軛 | 非共軛 | ||
| 反應(yīng)性 | 人、大鼠、小鼠、豬 | ||
| 應(yīng)用 | 蛋白質(zhì)印跡、免疫組織化學(xué)、免疫細(xì)胞化學(xué)、免疫組織化-石蠟切片、免疫組織學(xué)-冷凍切片、免疫組化-游離漂浮切片 | ||
| 宿主動(dòng)物 | 兔 | ||
| 抗體形式 | 血清凍干 | ||
| 產(chǎn)品形式 | 凍干的抗血清（不含防腐劑） | ||
| 復(fù)溶 | 200µl雙蒸餾水 | ||
| 產(chǎn)品描述 | Rabbit anti-Calbindin D-28k該抗血清是針對(duì)重組大鼠鈣結(jié)合蛋白D-28k（CB）產(chǎn)生的。它與許多其他物種的鈣結(jié)合蛋白D-28k發(fā)生交叉反應(yīng)，包括人類、猴子、大鼠、小鼠、雞和魚類。在免疫印跡中，它識(shí)別大約27-28kDa的單個(gè)條帶。在相關(guān)蛋白鈣視網(wǎng)膜蛋白濃度非常高（比CB高10倍）的樣品中，蛋白質(zhì)印跡中可能出現(xiàn)更大的條帶（29-30kDa）。因此，根據(jù)抗血清的稀釋度和/或免疫染色的敏感性，也可以揭示這種其他鈣結(jié)合蛋白。如有疑問，我們建議改用針對(duì)鈣結(jié)合蛋白D-28k的Swant單克隆抗體300，該抗體不識(shí)別鈣視網(wǎng)膜蛋白。 | ||
| 稀釋 | 免疫組織化學(xué)：抗生Wu素-生Wu素法1:10'000-1:20'00 免疫印跡：1:100-1:1’000。 我們建議通過滴定實(shí)驗(yàn)來確定稀釋度。 | ||
| 儲(chǔ)存 | 制作小份（例如2-5µl）。對(duì)于長(zhǎng)期儲(chǔ)存，應(yīng)保持在-80°C（或至少-20°C）。連續(xù)使用時(shí)，保持在4°C（含0.01%疊Dan化鈉）。避免反復(fù)冷凍和解凍。 | ||
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