Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)
Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)
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名稱 | 貨號(hào) | 英文名、縮寫 | 分子量 | 規(guī)格 | 價(jià)格 |
FITC-PEG-DBCO | PS2-FTDB-5K | FITC-PEG-DBCO | 5000 | 0.1g、0.5g | 現(xiàn)詢 |
FITC-PEG-DBCO | PS2-FTDB-2K | FITC-PEG-DBCO | 2000 | 0.1g、0.5g | 現(xiàn)詢 |
FITC-PEG-DBCO | PS2-FTDB-3K | FITC-PEG-DBCO | 3400 | 0.1g、0.5g | 現(xiàn)詢 |
FITC-PEG-DBCO | PS2-FTDB-1K | FITC-PEG-DBCO | 1000 | 0.1g、0.5g | 現(xiàn)詢 |
FITC-PEG-DBCO | PS2-FTDB-10K | FITC-PEG-DBCO | 10000 | 0.1g、0.5g | 現(xiàn)詢 |
?（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）
結(jié)構(gòu)式：
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基本信息：
=========================================?中文名稱：Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)
別名：FITC-PEG-DBCO
英文名稱：FITC-PEG-DBCO，F(xiàn)luorescein-PEG-DBCO
CAS：-
分子量：400、600、1K、2K、3.4K、4K、5K、10K
純度：90%以上
應(yīng)用簡(jiǎn)介：
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DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Copper-free Click Reaction Procedures:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
物理性質(zhì)：
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狀態(tài)：淡黃色到黃色固體，或者半固體，取決于分子量。
溶解性：溶于DMSO,DMF等溶劑。
保持條件：-20℃*保存，避光，干燥。
注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。
反應(yīng)性：DBCO于含有N3基團(tuán)的反應(yīng)。
技術(shù)指標(biāo)：
=========================================?純度（PEG）：90%以上
取代率（DBCO）：90%以上
取代率（FITC）：90%以上
分散系數(shù)（PDI）：小于等于1.05
分析：COA、GPC、NMR、MALDI-TOF
Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)
Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)
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