上海研盟生物科技有限公司Anti-Perilipin-2抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-Perilipin-2 antibody
中文名稱:脂滴包被蛋白抗體
脂滴包被蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
脂滴包被蛋白抗體,Anti-Perilipin-2抗體抗原修復(fù)方法:
方法1:沸水浴修復(fù),將盛有修復(fù)液和玻片的燒杯置于沸水浴環(huán)境,保持外部沸騰狀態(tài)15min,自然冷卻至室溫。
方法2:微波修復(fù),將盛有修復(fù)液和玻片的燒杯置于微波爐中,高火5min,?;?min,中火5min,自然冷卻至室溫。
方法3:高壓修復(fù),修復(fù)液加入高壓鍋加熱至沸騰,放入玻片,封蓋加壓持續(xù)加熱至噴氣時(shí)開始計(jì)時(shí)修復(fù)2min,自然冷卻至室溫。
方法4:使用*修復(fù),將*修復(fù)液加入到組織上,37℃,消化30min
★研盟生物★脂滴包被蛋白抗體,Anti-Perilipin-2抗體
Western Blotting 實(shí)驗(yàn)步驟概述:
1、 組織取材:組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液(每克組織3ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),
利用Polytron進(jìn)一步勻漿(15,000轉(zhuǎn)/分*1分鐘)維持4℃
4、 加入PMSF(每克組織30μl,10 mg/ml PMSF),冰上孵育30分鐘
5、 移入離心管4℃,約20,000g(約15,000轉(zhuǎn))15分鐘
6、 上清液為細(xì)胞裂解液可分裝-20℃保存
7、 進(jìn)行Bradford比色法測(cè)定蛋白質(zhì)濃度
8、 取相同質(zhì)量的細(xì)胞裂解液(體積*蛋白質(zhì)濃度),并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳(濃縮膠20mA,分離膠35mA)
12、電轉(zhuǎn)膜儀轉(zhuǎn)膜(100mA 40分鐘)
13、膜用麗春紅染色,膠用考馬斯亮藍(lán)染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
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相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
簡(jiǎn)單介紹:
產(chǎn)品別名:ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; ADRP; MGC10598; Perilipin-2; PLIN2; PLIN2_HUMAN.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
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CD68(to Mouse, rat) CD68抗體(用于大鼠、小鼠)
CD68(to human,bovine) CD68抗體(、牛)
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CD74/MHCⅡ(HLA class II histocompatibility antigen gamma chain ) CD74抗體
CLEC2D/OCIL(C-type lectin domain family 2 member D) CLEC2D蛋白抗體
CD80/B7-1 刺激分子B7-1蛋白抗體
CD71/TFR(Transferrin Receptor) 轉(zhuǎn)鐵蛋白受體抗體
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CD84/SLAMF5(SLAM family member 5) CD84抗體
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