上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-Pan Cytokeratin抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
廣譜細(xì)胞角蛋白PCK抗體,Anti-Pan Cytokeratin抗體簡單介紹:
【中文名稱】:廣譜細(xì)胞角蛋白PCK抗體
【英文名稱】:Anti-Pan Cytokeratin抗體
【英文別名】:pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1,2,4,5,6,7,8,71,72,75,78].
【規(guī) 格】:0.1ml/0.2ml
【相關(guān)標(biāo)記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.【濃 度】:1mg/1ml
【抗體來源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
【產(chǎn)品類型】:一抗
【產(chǎn)品應(yīng)用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細(xì)胞術(shù)等實(shí)驗(yàn)。
廣譜細(xì)胞角蛋白PCK抗體【背景介紹】:Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
廣譜細(xì)胞角蛋白PCK抗體,Anti-Pan Cytokeratin抗體免疫組化操作流程:
(一) 儀器設(shè)備
1. 18cm不銹鋼高壓鍋或電爐或用微波爐.
2. 水浴鍋
(二) 試 劑
1. PBS緩沖液(ph7.2―7.4):NaC137mmol/L,KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L,
KH2PO4 1.4mmol/L.
2.0.01mol/L檸檬酸鈉緩沖液(CB,ph6.0,1000ml):檸檬酸三鈉3g,檸檬酸0.4g。
3.0.5mol/L EDTA緩沖液(ph8.0):700ml水中溶解186.1g EDTA&
8226;2H2O,用10mmol/L
NaOH調(diào)至ph8.0,加水至1000ml.
4. 1mol/L的TBS緩沖液(ph8.0):在800ml水中溶解121gTris堿,用1N的HCl調(diào)至ph8.0,加水1000ml。
5. 酶消化液:a. 0.1%*:用0.1%CaCl 12(ph7.8)配制。b.0.4%*液:用0.1N的HCl配制。
6. 3%甲醇―H2O2溶液:用30%H2O2和80%甲醇溶液配制
7. 風(fēng)裱劑:a. 甘油和0.5mmol/L碳酸鹽緩沖液(ph9.0–9.5)等量混合
b 油和TBS(PBS)配制
8.TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標(biāo)本;ph7.0-7.4適合光學(xué)纖維標(biāo)本。
(三) 操作流程
1 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
★研盟生物 ★合作: ★客服
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
相關(guān)優(yōu)質(zhì)產(chǎn)品:
Cyclin A 周期素A抗體
Cyclin B1 周期素B1抗體
Cyclin C 周期素C抗體
Cyclin D1 周期素D1抗體
Cyclin D2 周期素D2抗體
Cyclin D3(human, mo, rat, pig, cattle, dog, Rb) 周期素D3抗體
Cyclin E 周期素E/原癌基因抗體
Cyclin F 周期素F抗體
Cyclin G 周期素G抗體
Cyclin H 周期素H抗體
Cygb (cytoglobin) 細(xì)胞球蛋白抗體
CYP1A1(Cytochrome p450 1A1) 細(xì)胞色素P450 1A1抗體
P450 1A2(Cytochrome P450 1A2) 細(xì)胞色素P450 1A2抗體
CYP3A4(Cytochrome p450 3A4) 抗細(xì)胞色素P450 3A4抗體
CYP19(Cytochrome p19) 細(xì)胞色素P450 19抗體
CYP21(Cytochrome P450 21; cytochrome P450, family 21, subfamily A, polypeptide 2) 21-羥化酶抗體
CYP450(Cytochrome P450 monooxygenase) 細(xì)胞色素P450單氧化酶抗體
Cyr61/IGFBP10/CCN1(cysteine rich 61) 富半*肝素結(jié)合蛋白61抗體
CYP2E1(cytochrome P450 2E1) 抗細(xì)胞色素P450ⅡE1抗體
Cytochrome C 細(xì)胞色素 C抗體
DRD1 receptor (dopamine D1 receptor) 多巴胺受體-D1抗體
DRD2 receptor(dopamine D2 receptor) 多巴胺受體-D2抗體
DRD3 receptor(dopamine D3 receptor) 多巴胺受體-D3抗體