中文名稱:宮頸黏液蛋白相關(guān)蛋白抗體
英文名稱:Anti-HIP55 antibody
上海研盟生物科技有限公司Anti-HIP55抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取。客服:.
宮頸黏液蛋白相關(guān)蛋白抗體,Anti-HIP55抗體簡(jiǎn)單介紹:
產(chǎn)品別名:ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; Src Homology 3 Domain Containing Protein; src homology 3 domain containing protein HIP.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說(shuō)明書(shū):請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來(lái)源:該指標(biāo)有兩種產(chǎn)品,一種是兔來(lái)源抗體,一種是鼠來(lái)源抗體
克隆類型:兔來(lái)源為多克隆抗體,鼠來(lái)源單克隆抗體
交叉反應(yīng):請(qǐng)索取說(shuō)明書(shū)查看
背景介紹:Drebrins (developmentally regulated brain proteins) are cytoplasmic proteins that bind F-actin in the brain and are involved in cell migration, extension of neuronal processes and plasticity of dendrites. HIP-55 (HPK1-interacting protein of 55 kDa), also known as ABP1, SH3P7 or DBNL (drebrin-like), is a 430 amino acid cytoplasmic protein that belongs to the ABP1 family. HIP-55 binds to F-actin but is not involved in actin polymerization, capping or bundling. In addition to containing an ADF-H domain, HIP-55 also consists of a SH3 domain, which mediates interaction with SHANK2, SHANK3 and PRAM-1. HIP-55 acts as an actin-binding adapter protein and as a common effector of antigen receptor-signaling pathways in leukocytes. As a key component of the immunological synapse, HIP-55 regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. HIP-55 is degraded by caspases during apoptosis.
宮頸黏液蛋白相關(guān)蛋白抗體保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
免疫應(yīng)答過(guò)程:
1.抗原進(jìn)入機(jī)體;
2.抗原刺激 B淋巴細(xì)胞,使之分化增殖成為抗體分泌漿細(xì)胞和記憶細(xì)胞(初次免疫應(yīng)答);
3.抗體主要由漿細(xì)胞分泌 ;
4.記憶細(xì)胞再次受到特異性抗原刺激后會(huì)被激活而成為分泌抗體的漿細(xì)胞(再次免疫應(yīng)答);
5.記憶B細(xì)胞是長(zhǎng)命細(xì)胞,可以存活數(shù)月甚至機(jī)體終生 。
初次體液免疫是指宿主*次接觸外來(lái)TD抗原后血清中逐漸出現(xiàn)抗原特異性抗體的過(guò)程。初次體液免疫應(yīng)答的延遲相(從抗原免疫到抗體水平達(dá)到高峰的時(shí)間)為6-10天,起初幾天血清中的抗原特異性抗體主要為親和力較低的IgM,后期以IgG為主。初次應(yīng)答過(guò)程的血清中抗原特異性抗體的高峰濃度較低且維持時(shí)間較短。再次體液免疫應(yīng)答是指曾被某抗原免疫過(guò)的宿主再次接觸該抗原時(shí)血清中迅速出現(xiàn)抗原特異性抗體的過(guò)程,其延遲相僅為4-5天,血清抗體以IgG為主,其高峰濃度較高且維持時(shí)間較長(zhǎng)。再次應(yīng)答所產(chǎn)生的抗體還有親和力成熟現(xiàn)象,即該抗體與抗原的親和力明顯高于初次應(yīng)答產(chǎn)生的抗體。
宮頸黏液蛋白相關(guān)蛋白抗體,Anti-HIP55抗體相關(guān)產(chǎn)品:
HEV 戊型肝炎病毒抗體
HGF(hepatocyte growth factor) 肝細(xì)胞生長(zhǎng)因子抗體
HGV(Hepatitis G vivus) 庚型肝炎病毒抗體
ZEBRA/HHV4/EBV(Human herpesvirus 4 type 2/epstein-barr virus ) 類皰疹病毒4抗體
HHV8/ORF K2(Human herpesvirus 8) 類皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8) 類皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HHV8/ORF50(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HHV8/ORF K2(Human herpesvirus 8 type P) 類皰疹病毒8抗體
HIF-1α (YMpoxia-inducible factor -1α) 缺氧誘導(dǎo)因子1α 抗體
HIF-1β/ARNT1(YMpoxia-inducible factor -1β) 缺氧誘導(dǎo)因子1β 抗體
HIF-2α (YMpoxia-inducible factor-2α) 缺氧誘導(dǎo)因子2α 抗體
HINT1(histidine triad nucleotide binding protein 1) *三聚體核苷結(jié)合蛋白1抗體
Histone H3,Family 3A (HISTIH3I protein) 抗組蛋白3抗體
Histone H1 抗組蛋白H1抗體
Histone H1b/Histone H1.4(Ac-Lys53) 抗乙酰化組蛋白H1b抗體
Histone H2/HTB901 抗組蛋白H2抗體
Histone H3-like protein 組蛋白H3樣抗體(Brassica juncea)
Histone H3-like protein 組蛋白H3樣抗體(Brassica junceaC端抗體)
Histone H3-like protein 組蛋白H3樣蛋白抗體(Brassica juncea N端抗體)
免疫組化操作步驟:
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無(wú)水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片