中文名稱：RIT1蛋白抗體

英文名稱：Anti-RIT1 antibody

RIT1蛋白抗體,Anti-RIT1抗體背景介紹：Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
Neuronal activity dramatically increases the concentration of cytosolic Ca2+, which then serves as a second messenger to direct diverse cellular responses. Calmodulin is a primary mediator of Ca2+ signals in the nervous system. Ric, a protein related to the Ras subfamily of small GTPases, has the ability to bind calmodulin. In addition, two Ras-like human proteins, Rin and Rit (Ric-related gene expressed in many tissues), which are 71% and 66% identical to RIC respectively, share related G2 domains with Ric. While most members of the Ras subfamily are plasma membrane-associated and generally require a C-terminal isoprenyl group to bind to the plasma membrane, Rit and Rin lack the recognition signal for C-terminal prenylation. Transiently expressed Rit and Rin are plasma membrane-localized because both proteins contain a C-terminal cluster of basic amino acids, which provides a mechanism for membrane association. Rin binds calmodulin through a C-terminal binding motif. Rit and Ric are widely expressed, whereas expression of Rin is restricted to the neuron system. In conclusion, Rit and Rin define a novel subfamily of Ras-related proteins

上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-RIT1抗體，我公司銷售國(guó)內(nèi)外科研產(chǎn)品，如生物試劑、生化試劑，一抗二抗，各種標(biāo)記抗體，Elisa酶聯(lián)免疫試劑盒，放免試劑盒，培養(yǎng)基，各種動(dòng)物血清等產(chǎn)品，歡迎廣大新老客戶前來咨詢訂購(gòu)，訂購(gòu):或。

相關(guān)標(biāo)記抗體：HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體

保質(zhì)期： 1年

RIT1蛋白抗體,Anti-RIT1抗體簡(jiǎn)單介紹：

產(chǎn)品別名：GTP binding protein Roc1; GTP-binding protein Rit1; Ras like protein expressed in many tissues; Ras like without CAAX 1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIBB; Ric like expressed in many tissues; RIT; RIT1; RIT1_HUMAN; ROC1.  

抗體來源：Rabbit or Mouse

克隆類型：Polyclonal or monoclonal

性    狀：Lyophilized or Liquid

濃    度：1mg/1ml

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

RIT1蛋白抗體抗原抗體反應(yīng)：
一、抗原抗體反應(yīng)特點(diǎn)
1.高度特異性，特異性是抗原抗體反應(yīng)的zui主要特征，特定抗原決定簇只與特定抗體結(jié)合；
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為：Ag+AbAg·Ab；
3.適當(dāng)濃度和比例；
4.敏感性:在測(cè)定血清中某一物質(zhì)的含量時(shí)，化學(xué)比色法的敏感度為mg/ml水平，酶反應(yīng)測(cè)定法的敏感度約為5~10μg/ml，免疫測(cè)定中凝膠擴(kuò)散法的敏感度與酶反應(yīng)法相仿。
二、影響抗原抗體反應(yīng)的因素
1.電解質(zhì) ：抗原抗體特異結(jié)合后，膠體粒便可發(fā)生脫水作用，此時(shí)易受電解質(zhì)影響，如有適當(dāng)濃度的電解質(zhì)存在就會(huì)使它們失去部分負(fù)電荷而相互凝聚，于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應(yīng)zui適溫度。

免疫組化染色失敗的幾種原因

1.所染的全部切片均為陰性結(jié)果，包括陽性對(duì)照在內(nèi)，全部呈陰性反應(yīng)，原因可能是：（1）染色未按嚴(yán)格操作步驟進(jìn)行；（2）漏加一種抗體，或抗體失效；（3）緩沖液內(nèi)含*，抑制了酶的活性；（4）底物中所加H2O2量少或失效；復(fù)染或脫水劑使用不當(dāng)。

2.所有切片均呈弱陽性反應(yīng)：（1）切片在染色過程中抗體過濃，或干燥了；（2）緩沖液配制中未加氯化鈉或pH不準(zhǔn)確，洗滌不*；（3）使用已變色的呈色底物溶液，或呈色反應(yīng)時(shí)間過長(zhǎng)；（4）抗體溫育的時(shí)間過長(zhǎng)；（5）H2O2濃度過高，呈色速度過快；（6）粘附劑太厚。

3.所有切片背景過深：（1）未用酶消化處理切片；（2）切片或涂片過厚；（3）漂洗不夠；（4）底物呈色反應(yīng)過久；（5）蛋白質(zhì)封閉不夠或所用血清溶血；（6）使用全血清抗體稀釋不夠。

4.陽性對(duì)照染色良好，檢測(cè)的陽性標(biāo)本呈陰性反應(yīng)，固定和處理不當(dāng)是zui常見的原因。對(duì)于陽性結(jié)果的定量判斷，常規(guī)方法是根據(jù)呈色深淺和陽性細(xì)胞數(shù)量分類計(jì)數(shù)，以（-）、+、++、+++等分級(jí)和計(jì)數(shù)統(tǒng)計(jì)。

新學(xué)期開學(xué)大減價(jià)*：

Anti-C5R1 補(bǔ)體成分5受體1抗體
Anti-TrkA/B/C *激酶A/B/C抗體
Anti-TrkA/NTRK1 *激酶A抗體
Anti-TRIM32/BBS11 神經(jīng)干細(xì)胞抑制相關(guān)蛋白TRIM32抗體
Anti-omerase Binding Protein, P23 端粒酶結(jié)合蛋白P23抗體
Anti-ABLIM3 肌動(dòng)蛋白結(jié)合蛋白LIM3抗體
Anti-TRF2 端粒結(jié)合蛋白2抗體
Anti-OLIG3 少突膠質(zhì)細(xì)胞轉(zhuǎn)錄因子3抗體
Anti-5HT3D+5HT3E Receptor 5-羥色胺受體3D+3E抗體
Anti-Transferrin 轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體
Anti-TRBF1/TRF1 端粒體復(fù)制結(jié)合因子1抗體
Anti-PCDHGA11 原鈣粘蛋白γA11抗體
Anti-Transthyretin/Prealbumin 轉(zhuǎn)甲狀腺素蛋白抗體
Anti-Desmoglein 2 橋粒芯糖蛋白2抗體
Anti-TRAIL 腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體抗體
Anti-Nfasc186 神經(jīng)束蛋白186抗體
Anti-TRAF6 腫瘤壞死因子受體相關(guān)因子6抗體
Anti-MOBP 少突膠質(zhì)細(xì)胞髓鞘相關(guān)蛋白抗體
Anti-TRAF3/CD40bp 腫瘤壞死因子受體相關(guān)蛋白3抗體
Anti-Cytokeratin 10+14+17+19+42 細(xì)胞角蛋白19，17，14，42，10抗體