上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-ABHD13抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:水解酶結(jié)構(gòu)域蛋白13抗體
英文名稱:Anti-ABHD13 antibody
保質(zhì)期: 1年
水解酶結(jié)構(gòu)域蛋白13抗體,Anti-ABHD13抗體背景介紹:The alpha/beta hydrolase superfamily comprise diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical alpha/beta hydrolase fold, which consists of an eightstranded parallel alpha/beta structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer’s disease, obesity and blood clotting disorders. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. Most of which utilize a serine nucleophile to form the G-X-S-X-G nucleophile elbow. ABHD1 plays a role in metabolizing smoking xenobiotics. ABHD2 participates in the development of atherosclerosis. ABHD4 is involved in an alternative synthesis pathway of NAE. Mutations in ABHD5 contribute to Chanarin-Dorfman syndrome. ABDH6 may play a role in nervous system metabolism and signaling. ABHD13 is a 337 amino acid single-pass membrane protein that belongs to the ABHD family.
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
水解酶結(jié)構(gòu)域蛋白13抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了??贵w溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補(bǔ)足,置于-20℃保存,這樣抗體不會(huì)凍,有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí),再使用對應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(*),至少三年有效。
水解酶結(jié)構(gòu)域蛋白13抗體,Anti-ABHD13抗體簡單介紹:
產(chǎn)品別名:ABHD13; ABHDD_HUMAN; abhydrolase domain containing 13; Abhydrolase domain-containing protein 13; BEM46L1; C13orf6.
抗體來源:Rabbit or Mouse
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
免疫組化操作步驟:
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
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