上海研盟生物有限公司*品質(zhì)供應Anti-SPTBN2抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:非紅細胞血影腦蛋白β2/spectrin β III抗體
英文名稱:Anti-SPTBN2抗體
避免反復凍融,保質(zhì)期一年。
非紅細胞血影腦蛋白β2抗體,Anti-SPTBN2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
各種HRP酶標記成抗體的效價測定程序如下(如:羊抗鼠IgG-HRP的效價測定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測定OD值;
10、陽性OD值比對照值大3倍,為判斷效價稀釋度。
非紅細胞血影腦蛋白β2抗體,Anti-SPTBN2抗體簡單介紹
別名:Beta III spectrin; Beta-III spectrin; SCA 5; SCA5; Spectrin beta chain brain 2; Spectrin beta non erythrocytic 2; Spectrin non erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN 2; Spectrin beta chain, non-erythrocytic 2; Beta-III spectrin; Spinocerebellar ataxia 5 protein; SPTN2_HUMAN.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產(chǎn)品類型:一抗
此產(chǎn)品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
非紅細胞血影腦蛋白β2抗體背景介紹:Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin Alpha and spectrin Beta are present in erythrocytes, whereas spectrin Alpha II (also designated fodrin Alpha) and spectrin Beta I (also designated fodrin Beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin Beta III is highly homologous to both spectrin Beta I and spectrin Beta II. Western blot analysis shows that spectrin Beta III migrates at a higher molecular mass than predicted in the kidney. Spectrin Beta III is highly expressed in brain, kidney, pancreas, and liver, and at lower levels in lung and placenta. Specifically, spectrin Beta III constitutes a major component of the Golgi and vesicular membrane skeletons.
免疫熒光染色與免疫組化前期處理相同,因為內(nèi)源性過氧化物酶對免疫熒光實驗沒有影響,同時雙氧水反而會干擾到熒光素發(fā)光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個通路,激發(fā)波長為350nm、488nm、555nm附近,分別對應的熒光發(fā)射光顏色為藍光、綠光、紅光。
350nm常用熒光素:DAPI(復染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
非紅細胞血影腦蛋白β2抗體,Anti-SPTBN2抗體相關優(yōu)質(zhì)產(chǎn)品:
Mfn1 (Mitofusin1) 線粒體融合蛋白1抗體
MGMT (O6-metYMlguanine-DNA metYMltransferase) O6甲基*DNA甲基轉(zhuǎn)移酶抗體
MT(metallothionein) 金屬硫蛋白抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(NT) 層粘連蛋白受體1抗體(N端)
MTA1(Metastasis-associated 1) 腫瘤轉(zhuǎn)移相關蛋白1抗體
MGr1-Ag/37LRP(P37-kDa laminin receptor precuBYKor)(CT) 層粘連蛋白受體1抗體(C端)
Myf6(Myogenic factor 6) 生肌調(diào)節(jié)因子6抗體
MGr1-Ag monoclonal antibody(laminin receptor 1; 67kD, ribosomal protein SA) 小鼠抗層粘連蛋白受體1單克隆抗體
MICA(MHC class I polypeptide-related sequence A) MHC I類鏈相關蛋白A抗體
Microsporidia 蜜蜂微孢子蟲蛋白抗體
Midnolin isoform Protein 1 中腦核仁蛋白1抗體
Midnolin isoform Protein 2 中腦核仁蛋白2抗體
MK(Midkine) 中期因子/肝素結(jié)合生長因子抗體
MLCK(myosin light chain kinase) 肌球蛋白輕鏈激酶抗體
MIF (Macrophage Migration Inhibitory Factor) 巨噬細胞移動抑制因子抗體
MIP-1α (macrophage inflammatory protein 1α) 巨噬細胞炎癥因子1α抗體
MIP-1β (macrophage inflammatory protein 1β) 巨噬細胞炎癥因子1β 抗體
MIP4/CCL18(Macrophage inflammatory protein 4) 巨噬細胞炎癥蛋白18抗體