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Anti Ras(Q61H) Mouse Monoclonal Antibody

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Anti Ras(Q61H) Mouse Monoclonal Antibody

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Ras(Q61H) [26195] 
[100ul]


Catalog Number:  26195
Gene Symbol:  RAS
Description:   Anti Ras(Q61H) Mouse Monoclonal Antibody
Background:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras Q61H mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of Q61H, human origin
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA 1:100-1:5000 
WB 1:100-1:2000 
IF 1:50-1:100 
IHC 1:50-1:100
Concentration:   2 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   Unknown
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Ras(Q61H) mutated, but not wild-type Ras proteins of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), Ras Q61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras Q61H mouse monoclonal antibody (Cat. #26195).
Immunofluorescence:  
Immunofluorescence of cells expressing Ras proteins with Anti Ras(Q61H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(Q61H) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with Anti Ras(Q61H) monoclonal antibody (Cat. #26195).


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Publications:
1.  
    Gene Therapy 21, 888-896 (October 2014)
2.  
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136




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