Anti β catenin(E53K) Mouse Monoclonal Antibody

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武漢紐斯特生物科技有限公司

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所在地區(qū)：湖北武漢市

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βcatenin(E53K)[26167][100µL]CatalogNumber:26167GeneSymbol:CTNNB1

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β catenin(E53K) [26167]
[100 µL]

Catalog Number: 26167

Gene Symbol: CTNNB1, CTNNB

Description: Anti β catenin(E53K) Mouse Monoclonal Antibody

Background: Beta-catenin (or β catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.

Immunogen: A synthetic peptide from the internal region of β catenin which includes the mutation of E53K, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes β catenin(E53K), but not wild type β catenin protein from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant β catenin(E53K) and wildtype proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti β catenin(E53K) mouse monoclonal antibody (Cat. #26167).

Immunofluorescence:

Immunofluorescence of cells expressing β catenin proteins with anti-β catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β catenin WT) plasmid (left column) or pCDNA3-GFP-β catenin(E53K) plasmid (right column), then fixed and stained with anti β catenin(E53K) monoclonal antibody (Cat. #26167).


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