MRC-5細(xì)胞
細(xì)胞名稱：人胚肺細(xì)胞
細(xì)胞簡稱：MRC-5
產(chǎn)品貨號：TCH-C263
種屬來源：人
組織來源：肺
疾病特征：/
細(xì)胞形態(tài)：成纖維細(xì)胞樣
生長特性：貼壁生長
培養(yǎng)體系： DMEM-H+10%FBS+1%P/S
配套培養(yǎng)基貨號：TCH-G263
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存
質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性
MRC-5細(xì)胞是一種常用的人類胚肺細(xì)胞，是由1966年從一個正常的男性胎兒的肺部組織獲得，具有非常良好的細(xì)胞生長和增殖特性，可廣泛應(yīng)用于細(xì)胞培養(yǎng)、病毒研究和毒性測試等領(lǐng)域。
該細(xì)胞系來自14周齡男性胎兒的正常肺組織，該細(xì)胞老化前能傳代42~46個倍增時(shí)間。
參考文獻(xiàn)：
Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953
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