Hep3B2.1-7 人肝癌細胞

    Hep-3b細胞建系于1979年，源自一位患有肝癌的7歲黑人男童。該細胞產(chǎn)生甲胎蛋白（alpha-fetoprotein)、乙肝表面抗原（BHsAg)、白蛋白、巨球蛋白、α-抗（alphal-antitrypsin)、轉(zhuǎn)鐵蛋白、補體（C3)、C3活性載體，纖維原等，具有廣泛的研究價值。

細胞名稱： Hep3B2.1-7 人肝癌細胞

細胞簡稱：Hep3B2.1-7

產(chǎn)品貨號：TCH-C195

種屬來源：人

組織來源：肝臟

特征：肝癌

細胞形態(tài)：上皮細胞樣

生長特性：貼壁生長

培養(yǎng)體系：MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S

人肝癌細胞  Hep3B2.1-7培養(yǎng)基貨號：TCH-G195

傳代比例：1:2-1:3，每2-3天換液一次

傳代周期：72-96 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細菌、真菌、支原體檢測均為陰性

參考文獻：

Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137

Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983