Hut78細(xì)胞
細(xì)胞名稱： 人T淋巴細(xì)胞白血病細(xì)胞（STR鑒定正確）
細(xì)胞簡(jiǎn)稱：Hut78細(xì)胞
細(xì)胞別名： Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;
HUT78; NCI-H78
貨號(hào)：TCH-C218
規(guī)格：1×10?cells/ T25 培養(yǎng)瓶
價(jià)格：1400元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來(lái)源：人
組織來(lái)源：皮膚
特征：塞扎里綜合癥
細(xì)胞形態(tài)：淋巴母細(xì)胞
生長(zhǎng)特性：懸浮生長(zhǎng)
培養(yǎng)體系：IMDM+20%FBS+1%P/S
細(xì)胞配套培養(yǎng)基 貨號(hào)：TCH-G218
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存
質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性
NCI-H78 細(xì)胞 可產(chǎn)生IL-2，TNF-α；表達(dá)IL-2受體
參考文獻(xiàn)：
Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346
Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177
Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013
Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674
O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285
Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082
Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.
Cancer Cell 31:225-239(2017)
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