L-929細胞-小鼠成纖維細胞
細胞名稱：L-929細胞-小鼠成纖維細胞
細胞別稱： NCTC 929; NCTC-929; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC);
Clone 929; Earles's cells; Earle's L cells
規(guī)格： 1X10^6| T25培養(yǎng)瓶
價格：1500元 (干冰運輸需另加200元干冰費)
種屬來源：小鼠
組織來源：皮下結締組織
細胞形態(tài)：成纖維細胞樣
生長特性：貼壁生長
培養(yǎng)體系：MEM+10%FBS+1%P/S
L-929細胞-小鼠成纖維細胞 配套培養(yǎng)基貨號：TCM-G749
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養(yǎng)基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
1948年三月建立了NCTCclone929(小鼠結締組織)，細胞系L的克隆。細胞系L是較早建立的連續(xù)培養(yǎng)細胞系之一，而clone929是較早的克隆株。從一只100日齡的雄性C3H/An小鼠的正常皮下疏松結締組織入脂肪組織中建立了親本細胞系L。第95代的細胞系L使用毛細管法分離單細胞建立了clone929。
參考文獻：
"Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324
Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517
Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311
ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.
ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).
U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed:
Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."
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