MHCC97H-人肝癌細(xì)胞
細(xì)胞名稱(chēng):MHCC97H-人肝癌細(xì)胞 (STR鑒定正確)
細(xì)胞別名: MHCC 97-H; MHCC-97H; MHCC97H
細(xì)胞簡(jiǎn)稱(chēng):MHCC97H
產(chǎn)品貨號(hào):TCH-C258
產(chǎn)品規(guī)格: 1×10?cells/T25 培養(yǎng)瓶
種屬來(lái)源:人
組織來(lái)源:肝
特征:高轉(zhuǎn)移潛能肝癌細(xì)胞
細(xì)胞形態(tài):上皮細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系:DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
MHCC97H-人肝癌細(xì)胞 配套培養(yǎng)基貨號(hào):TCH-G258
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
推薦使用T25瓶,復(fù)蘇發(fā)貨1-2周
MHCC97H-人肝癌細(xì)胞
參考文獻(xiàn):
1. Dang H., Steinway S.N., Ding W., Rountree C.B.
Induction of tumor initiation is dependent on CD44s in c-Met(+) hepatocellular carcinoma.
BMC Cancer 15:161-161(2015)
2. Tian M.-M., Cheng H., Wang Z.-Q., Su N., Liu Z.-X., Sun C.-Q., Zhen B., Hong X.-C., Xue Y., Xu P.
Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H.
Int. J. Mol. Sci. 16:4209-4225(2015)
3. Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)
4. Li Y., Tian B., Yang J., Zhao L., Wu X., Ye S.-L., Liu Y.-K., Tang Z.-Y.
Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics.
J. Cancer Res. Clin. Oncol. 130:460-468(2004)
5. Ding S.-J., Li Y., Shao X.-X., Zhou H., Zeng R., Tang Z.-Y., Xia Q.-C.
Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials.
Proteomics 4:982-994(2004)
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