Mouse Anti- GPR30單抗說(shuō)明書(shū)
Catalog Number:Rs-1380R
Quantity size:0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: G protein-coupled receptors （GPRs, or GPCRs） contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GRP30 is a member of this family and is an orphan receptor. GPR30 expression has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. In brain, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. However, unlike other known G protein-coupled receptors, GPR30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives.
Mouse Anti- GPR30單抗說(shuō)明書(shū)
Specificity : ·anti-GPR30 is aMouse monoclonal antibody unconjugated ·specific for GPR30 of Rabbit
·use for western blot, elisa, immunoprecipitation
and immunohistochemistry
·affinity chromatography purification, purity :>95%
·isotype: IgG
·mol wt: 38kDa
Mouse Anti- GPR30單抗說(shuō)明書(shū)
Application :· Immunohistochemistry: 1:100-500 · ELISA: 1:500-1000
· Western blotting 1:100-500
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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