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更新時間：2024-02-10 17:41:51瀏覽次數(shù)：152次
聯(lián)系我時，請告知來自 環(huán)保在線不封端的丙基氰基；可被成功用于正相和反相分離，HSS CN是最多樣化的固定相。
XSelect HSS CN 柱是—款超高性能通用的丙基氰基鍵合相，提供*的峰形，增加保留，在低PH值條件下具有延長柱壽命。高強度硅膠顆粒（HSS）技術(shù)專為補充雜化BEH和CSH顆粒開發(fā)，用于提高色譜效率。高孔徑容量的硅膠基質(zhì)HPLC顆粒不具備必要的機械穩(wěn)定性來承受UPLC分離過程中的壓力，如果實驗室需要將現(xiàn)有的HPLC方法轉(zhuǎn)移到UPLC系統(tǒng)上的話，這一點就顯得尤為重要。機械穩(wěn)定性的限制促使沃特世的科學(xué)家設(shè)計出一種顆粒具有高機械穩(wěn)定性和較長的使用壽命以及高壓下的UPLC的柱效。
XSelectHSS CN色譜柱可被成功用于正相和反相分離，是最多樣化的固定相。
Determination of Soy Isoflavones in Foods and Dietary Supplements by UPLC
Isoflavones are a class of plant-derived compounds, produced almost exclusively by members of the Fabaceae family, that have been shown to have estrogenic activity in mammals. The major source of isoflavones in the human diet comes from soybeans, in which genistein and daidzein are the predominant components. Isoflavones remain the subject of many scientific studies with accepted methods established by organizations such as AOAC1 and USP2 , utilizing traditional reversed-phase C18 columns. Recently, the National Institute of Standards and Technology (NIST) has developed a suite of soy-based candidate Standard Reference Materials (cSRMs) for the certification of soy isoflavones in foods and dietary supplements. For the analysis of those standards, they have developed a method utilizing a 60-minute gradient on a 5 µm cyano column which enables the separation of the soy isoflavones from other components not resolved using the standard C18 methods.3 Using these conditions, they have demonstrated resolution of the three main soy isoflavones and their glycosides (structures shown in Figure 1), as well as the acetyl and malonyl conjugates of the glycosides. Since the conjugates are somewhat unstable in solution, they have added an additional sample preparation step to hydrolyze these conjugates to their associated glycoside, providing a more accurate, reproducible determination of total isoflavone content. Optimization of this method utilizing the XSelect HSS Cyano XP 2.5 µm Column provides a significant reduction in analysis time by traditional HPLC. Further optimization of this method to UPLC® with the ACQUITY UPLC HSS Cyano 1.8 µm Column provides additional savings in time, while maintaining the resolution for accurate determination of soy isoflavones.
品名 | 編號 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 1 mm X 100 mm, 1/pkg | 186005903 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 1 mm X 150 mm, 1/pkg | 186005904 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186005906 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186005907 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 2.1 mm X 75 mm, 1/pkg | 186005908 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186005909 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186005910 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 3 mm X 100 mm, 1/pkg | 186005915 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 3 mm X 30 mm, 1/pkg | 186005912 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186005913 |
XSelect HSS Cyano (CN) Column, 100?, 3.5 µm, 3 mm X 150 mm, 1/pkg | 186005916 |
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