日本一個(gè)研究小組日前發(fā)現(xiàn)，在雌鳉魚的卵巢內(nèi)存在可發(fā)育成鳉魚卵的干細(xì)胞。有關(guān)成果已經(jīng)刊登在一期的美國(guó)《科學(xué)》雜志網(wǎng)絡(luò)版上。

這一成果是日本自然科學(xué)研究機(jī)構(gòu)基礎(chǔ)生物學(xué)研究所的研究人員獲得的?；A(chǔ)生物學(xué)研究所日前發(fā)表公告說，在脊椎動(dòng)物中，已經(jīng)發(fā)現(xiàn)雄性的精巢中存在發(fā)育成精子的干細(xì)胞，但是在卵巢中發(fā)現(xiàn)會(huì)發(fā)育成卵的干細(xì)胞則屬世界。

研究小組對(duì)雌性鳉魚進(jìn)行基因操作，使其卵巢內(nèi)的卵發(fā)出紅光，結(jié)果發(fā)現(xiàn)了很多即將發(fā)育為卵的細(xì)胞。利用綠色熒光蛋白質(zhì)給這些細(xì)胞做上標(biāo)記以后，研究人員發(fā)現(xiàn)許多新形成的卵也會(huì)發(fā)出綠色熒光，這說明這些細(xì)胞的確發(fā)育成了卵，因此這些細(xì)胞就是干細(xì)。

鳉魚等魚類會(huì)大量產(chǎn)卵，但是詳細(xì)機(jī)制一直沒有弄清。研究小組成員田中實(shí)指出，他們的新發(fā)現(xiàn)可能為開發(fā)的養(yǎng)殖技術(shù)作出貢獻(xiàn)。

Science DOI: 10.1126/science.1185473

Identification of Germline Stem Cells in the Ovary of the eost Medaka
Shuhei Nakamura,1 Kayo Kobayashi,1 Toshiya Nishimura,1,2 Shin-ichi Higashijima,3,4 Minoru Tanaka1,2,*

Germline stem cells continually produce sperm in vertebrate testes, whereas there is no direct evidence showing that germline stem cells are present in adult vertebrate ovaries. Using transgenic methods and clonal analysis, we identified germline stem cells that supported oogenesis and the production of offspring in the ovaries of adult medaka fish. Early-stage germ cells were localized in clusters along interwoven threadlike cords of sox9b-expressing somatic cells （termed "germinal cradles"） where the germ cells developed. Germline stem cells gave rise to germ cells that divided to produce cysts, which then underwent cell death or separated to form follicles. Our results provide insight into germline stem cell biology of medaka and provide a model system for studying vertebrate stem cell niches.

1 Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan.
2 Department of Basic Biology, Graduate University for Advanced Studies （SOKENDAI）, Okazaki, Aichi 444-8585, Japan.
3 National Institutes of Natural Sciences, Okazaki Institute for Integrative Bioscience, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
4 Department of Physiological Sciences, Graduate University for Advanced Studies （SOKENDAI）, Okazaki, Aichi 444-8585, Japan.