大鼠緊密連接蛋白(Ocln) ELISA kit

產(chǎn)品類型:
ELISA Kit

產(chǎn)品名稱:

大鼠緊密連接蛋白(Ocln) ELISA kit

英文名稱:

Rat Occludin (Ocln) ELISA kit

貨號:
大鼠緊密連接蛋白(Ocln) ELISA kit別名:
, tight junction protein occludin TM4 minus
規(guī)格:
96T
中文價格:
大鼠緊密連接蛋白(Ocln) ELISA kit種屬:
Rat
其他種屬:
Human
待測物名稱:
Occludin (Ocln)
縮寫:
Ocln
檢測范圍:
25 pg/ml-1600 pg/ml
靈敏度:
6.25 pg/ml
反應(yīng)時間:
1-5h
所需樣本體積:
50-100ul
檢測波長:
450 nm

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Ocln has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ocln present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody 大鼠緊密連接蛋白(Ocln) ELISA kitspecific for Ocln is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ocln bound in the initial step. The color development is stopped and the intensity of the color is measured.