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當(dāng)前位置:上海滬震生物科技有限公司>>技術(shù)文章>>豬三碘甲狀腺原氨酸(T3)ELISA Kit
豬三碘甲狀腺原氨酸(T3)ELISA Kit
產(chǎn)品類型:
ELISA Kit
96T
Pig
Tri-iodothyronine,T3
T3
Thyroid function
0.5ng/ml-8ng/ml
0.245ng/ml
1-5h
50-100ul
450 nm
豬三碘甲狀腺原氨酸(T3)ELISA Kit This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.
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