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Anti-β-catenin/β-cats 抗體說明書

時間:2011/11/15閱讀:724
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 Anti-β-catenin/β-cats 抗體說明書

 Cat. Number:  Rs-1165R  
Quantity size:  0.1ml/0.2ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background:  Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.
 

 Anti-β-catenin/β-cats 抗體說明書

Specificity:      · anti-β-catenin/β-cats is a rabbit monoclonal antibody unconjugated  
                ·specific for β-catenin/β-cats of human, mo, rat, chicken
                ·epitope mapping near the β-catenin/β-cats of human, mo, rat,
chicken
· use for western blotting, elisa, immunoprecipitation  
                   and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG  
· mol wt: 92kDa
 
Application:     ·Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-β-catenin/β-cats 抗體說明書

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