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上海瑞齊生物科技有限公司

Anti- GCS, FITC conjugated抗體說明書

時(shí)間:2011-12-20閱讀:385
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 Anti- GCS, FITC conjugated抗體說明書


Quantity size : 0.2ml (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium azide. Reconstitute with 0.2ml sterile distilled water. )
 
Background: GCS (glucosylceramide synthase ) may serve as a 'flippase' as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family.

 Anti- GCS, FITC conjugated抗體說明書


Specificity :
· anti- GCS is a rabbit polycional antibody, FITC conjugated
           · specific for GCS of human, mouse and rat  
           · epitope mapping near the amino terminus of GCS of human, mouse and rat origin  
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG 
· mol wt:48kDa

Application : · FCM: 1:20-100
            · Immunohistochemistry : 1:20-1:200
            · Immunocytochemistry: 1:20-1:200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
· Optimal working dilutions must be determined by the end user.
· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti- GCS, FITC conjugated抗體說明書

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