Mouse Anti-SOD抗體說(shuō)明書，現(xiàn)貨wb操作

FROM:abcam

Catalog Number : RS-1079R

Quantity size :0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
background:
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Specificity :

–anti-SOD is a mouse Polyclonal antibody.Anti-SOD抗體說(shuō)明書，現(xiàn)貨wb操作

– specific for anti-SOD antibody of human,Rat

– use for western blotting, elisa, immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

• Isotype: IgG
• mol wt:17 kDa

Application :

– Western blotting 1:100-500

– Immunohistochemistry 1:100-500

– ELISA 1:500-1000

– Optimal working dilutions must be determined by the end user.Anti-SOD抗體說(shuō)明書，現(xiàn)貨wb操作

Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

The lyophilized antibody is stable at room temperature for at least one month and for

greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M

PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃.

Important Note: This product as supplied is intended for research use only, not for

use in human, therapeutic or diagnostic applications.(用熒光色素標(biāo)記的抗體）
?試管標(biāo)號(hào)。
?試管內(nèi)加入 20 μl 熒光色素標(biāo)記的抗體。
?每個(gè)試管中加入 100 μl 準(zhǔn)備好的細(xì)胞懸液（相當(dāng)于 100 萬(wàn)細(xì)胞）。
?置于加蓋的冰盒內(nèi)，振蕩并孵育 15－30 分鐘。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗體。
?用臺(tái)式離心機(jī) 2000 RPM 離心 5 分鐘。如果是細(xì)胞內(nèi)染色，離心速度應(yīng)增加到 3000 或 4000 RPM。
?小心吸去上清液，勿將沉淀沖起。
?1% 多聚甲醛 500 μl 重懸浮細(xì)胞。試管可以存放在避光處 24 小時(shí)（細(xì)胞內(nèi)染色zui長(zhǎng)時(shí)間）到 1 周（表面染色zui長(zhǎng)時(shí)間）。
間接染色（用非熒光素標(biāo)記的一抗和熒光色素標(biāo)記的二抗）
?試管標(biāo)號(hào)。
?試管內(nèi)加入未標(biāo)記的一抗。每管大約 1 μg。
?每個(gè)試管中加入 100 μl 準(zhǔn)備好的細(xì)胞懸液（相當(dāng)于 100 萬(wàn)細(xì)胞）。
?置于加蓋的冰盒內(nèi)，振蕩并孵育 15－30 分鐘。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗體。
?用臺(tái)離心機(jī) 2000 RPM 離心 5 分鐘。如果是細(xì)胞內(nèi)染色，離心速度應(yīng)增加到 3000 或 4000 RPM。
?小心吸去上清液，勿將沉淀沖起。
?每管加入 100 μl 1x PBS。試管內(nèi)加入熒光色素標(biāo)記的二抗 0.5-1 μg。
?置于加蓋的冰盒內(nèi)，振蕩并孵育 15－30 分鐘。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗體。