The chelating effect of picolyl azide moiety to raise the effective of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the of the copper catalyst without sacrificing the efficiency of labeling.
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Abs/Em Maxima | 647/663 nm |
Extinction coefficient | 250,000 |
Flow cytometry laser line | 633 or 635 nm |
Microscopy laser line | 633 or 635 nm |
Spectrally similar dyes | Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649 |
Molecular weight | 953.15 |
CAS | N/A |
Solubility | Water, DMSO, DMF, MeOH |
Purity | >95% (HPLC) |
Appearance | Blue solid |
Storage conditions | -20C |
Shipping conditions | Ambient temperature |
