產(chǎn)品名稱:3d灌注流培養(yǎng)系統(tǒng)品牌:貨號: 價格:詢價:李先生 電話:

Quasi Vivo細胞灌流培養(yǎng)系統(tǒng)

英國Kirkstall公司生產(chǎn)的創(chuàng)新性細胞培養(yǎng)產(chǎn)品,其Quasi Vivo灌流培養(yǎng)系統(tǒng)可為細胞培養(yǎng)提供持久恒定的流動培養(yǎng)環(huán)境,zui大限度模擬體內(nèi)環(huán)境。相比各類傳統(tǒng)靜態(tài)培養(yǎng)系統(tǒng),Quasi Vivo流動培養(yǎng)系統(tǒng)更大限度模擬了細胞在體內(nèi)生長環(huán)境,其流動培養(yǎng)方式更利于培養(yǎng)基內(nèi)營養(yǎng)物質(zhì)的擴散和細胞代謝產(chǎn)物的運輸,更有利于復雜細胞模型的構(gòu)建,尤其適合需要在氣液界面進行分化的各類呼吸道上皮細胞的生長。

Quasi Vivoquan球應用

quan球使用Kirkstall公司Quasi Vivo灌流培養(yǎng)系統(tǒng)的學術(shù)及研究機構(gòu)已達70+個,遍布美國、英國、法國、瑞典、奧地利、意大利、荷蘭、瑞士、日本等。目前Quasi Vivo灌流培養(yǎng)系統(tǒng)已成功用于以下器官模型的培養(yǎng):

1. 呼吸系統(tǒng)(培養(yǎng)熱點)

2. 肝臟

3. 腎臟

4. 心血管

5. 成纖維細胞

6. 糖尿病模型

7. 血腦屏障

8. 腦組織類器官

典型應用例子:

灌流培養(yǎng)

呼吸道上皮細胞的氣液界面培養(yǎng)是研究經(jīng)空氣傳播的病原體,如SARS等的常用的模型。傳統(tǒng)的培養(yǎng)方式是用TransWell在普通培養(yǎng)箱中靜置培養(yǎng)。但是此種培養(yǎng)方式wu法模擬培養(yǎng)過程中營養(yǎng)物質(zhì)和代謝廢物在組織內(nèi)的運輸,培養(yǎng)得到的模型通常有各種各樣的缺陷,并且所需實驗周期較長。

呼吸道上皮細胞的常規(guī)transwell靜止培養(yǎng)方式

Quais Vivo(QV600)灌流培養(yǎng)系統(tǒng)(腔室+儲液瓶+底座+管道+泵等)

而Quasi Vivo灌流培養(yǎng)系統(tǒng)可為細胞培養(yǎng)提供持久恒定的流動培養(yǎng)環(huán)境,zui大限度模擬體內(nèi)環(huán)境。研究發(fā)現(xiàn),使用Quasi Vivo系統(tǒng)進行灌流培養(yǎng)與靜態(tài)培養(yǎng)相比,氣液界面培養(yǎng)的呼吸道上皮細胞(正常人氣管上皮細胞 Normal Human Bronchial Epithelial Cells,簡稱NHBE;小氣道上皮細胞 Small Airway Epithelial Cells,簡稱SAE),發(fā)育分化速度更快,表現(xiàn)為纖毛分化度更高,纖毛運動更強、粘液產(chǎn)生和屏障功能更強。在灌注下加速分化后,將上皮細胞轉(zhuǎn)移到靜態(tài)條件下,并添加抗原呈遞細胞(APC)以研究其在病原體感染后的功能。(Chandorkar P, et al., Fast-track development of an in vitro 3D lung/immune cell model to study Aspergillus infections. Sci Rep. 2017 7(1):11644. doi: 10.1038/s41598-017-11271-4.)

01、人體內(nèi)鎖有的細胞都需要營養(yǎng)物質(zhì)和代謝廢物的流動

02、肺部氣管/支氣管和小氣道上皮結(jié)構(gòu)精細,進行體外培養(yǎng)模擬體內(nèi)環(huán)境,對呼吸道病原體的研究至關(guān)重要

03、采用quan新的灌流培養(yǎng)方式培養(yǎng)呼吸道上皮細胞(采用QV600)

相比使用transwell靜止培養(yǎng)(Static Conditions),Quasi Vivo灌流培養(yǎng)系統(tǒng)(Perfused Conditions)中,呼吸道上皮細胞的生長和分化呈現(xiàn)更好狀態(tài)

04、電鏡照片顯示,采用灌流培養(yǎng)方式(Perfused conditions)的呼吸道上皮細胞,分化程度更高

05、使用MUC5B染色可以發(fā)現(xiàn),采用灌流培養(yǎng)方式(Perfused conditions)的呼吸道上皮細胞,在培養(yǎng)的弟7天即可分泌大量粘液。用OCCLUDIN染色可以發(fā)現(xiàn),細胞間的緊密連接發(fā)育更完善

06、使用WGA染色發(fā)現(xiàn),采用灌流培養(yǎng)方式(Perfused conditions)的呼吸道上皮細胞,纖毛分化度更高

07、測量TEER(經(jīng)細胞電阻),采用灌流培養(yǎng)方式(Perfused conditions)的呼吸道上皮細胞TEER值更大,代表得到的上皮細胞膜狀結(jié)構(gòu)更完整

一、不同細胞,Quasi Vivo型號怎么選?

:使管路上游的細胞培養(yǎng)基成為下游細胞的條件培養(yǎng)基。

流動培養(yǎng)形成含血管的3D心臟組織 | 再生醫(yī)學

在再生醫(yī)學領域,怎樣培養(yǎng)出含血管的組織,是未來應用能否成功的關(guān)鍵之一。早期的臨床試驗采用生長因子或細胞注射的方法來修補損傷的心臟,但由于注射細胞造成的炎癥反應和局部缺血會在體內(nèi)造成低氧環(huán)境,使得注射的細胞定植率低而死亡率高,不能有效地修復損傷的心臟功能。

Quasi Vivo QV500流動培養(yǎng)系統(tǒng)為接種在明膠支架上的人間充質(zhì)干細胞(hMSCs)和人心肌祖細胞(hCMPC)提供充足的氧氣,促進細胞和營養(yǎng)物質(zhì)向支架核心內(nèi)擴散,并能快速有效地排除組織內(nèi)的代謝廢物,促進血管生成,從而形成由血管樣和心臟樣細胞組成的組織結(jié)構(gòu)密集的適于體內(nèi)移植的原組織。(Pagliari S, et al. A multistep procedure to prepare pre-vascularized cardiac tissue constructs using adult stem cells, dynamic cell cultures, and porous scaffolds. Frontiers in Physiology. 2014; 5: 210)

Quasi-Vivo流動培養(yǎng)系統(tǒng) (QV500型)的蠕動泵將培養(yǎng)基從儲液瓶泵到兩個串聯(lián)的培養(yǎng)腔室內(nèi),并能保持恒定流速(200μl/min),保證多孔明膠支架內(nèi)層的培養(yǎng)基流動。

構(gòu)建含血管的3D心臟的實驗方案示意圖。明膠多孔支架被浸入稀釋的Matrigel中,然后轉(zhuǎn)移至內(nèi)皮分化培養(yǎng)基中。之后將人間充質(zhì)干細胞接種在支架上,使人間充質(zhì)干細胞定植在支架培養(yǎng)上并向內(nèi)皮進行分化,96小時后,將在聚苯乙烯細胞培養(yǎng)板用心臟分化培養(yǎng)基預先定型2周的心臟-GFP人心肌祖細胞接種于血管化的支架上,用QV500流動培養(yǎng)系統(tǒng)在心臟分化培養(yǎng)基中培養(yǎng)7天。

采用上述實驗方案,對用QV500培養(yǎng)一周后的共培養(yǎng)結(jié)構(gòu)進行檢測,發(fā)現(xiàn)在支架上有大量細胞定殖。

QV500流動培養(yǎng)條件下支架內(nèi)部浸潤了大量的血管樣細胞(紅色)和人心肌前體細胞(hCMPC)衍生的心肌細胞(綠色),而靜態(tài)培養(yǎng)條件下,細胞大部分分布在支架表面。

免疫組化結(jié)果顯示通過QV500動態(tài)培養(yǎng)可以促進心肌樣細胞(GFP,綠色)和內(nèi)皮樣細胞(VCAM-1陽性細胞,紅色)向支架內(nèi)部浸潤。

(A) 切片顯示QV500流動培養(yǎng)的內(nèi)皮樣細胞(VCAM-1陽性細胞,紅色)排列成孔狀,形成管狀結(jié)構(gòu),并與心肌樣細胞(GFP,綠色)接觸。(B)QV500流動培養(yǎng)條件下,支架內(nèi)廣泛的細胞分布導致形成密集組裝的多細胞組織,該組織衍生自所用的人間充質(zhì)干細胞(hMSCs)和人心肌前體細胞(hCMPC)。

總結(jié):在本文中使用的QV500流動培養(yǎng)系統(tǒng),能增強氧氣與營養(yǎng)物質(zhì)的運輸,進而增強工程化心血管組織的活性和功能。

與眾不同的Quasi Vivo流動培養(yǎng)系統(tǒng),讓日、美、英、法、瑞士、瑞典等quan球70多個研究機構(gòu)獲得了更強大的細胞培養(yǎng)工具,在包括呼吸系統(tǒng)、心血管系統(tǒng)、肝臟、腎臟、腸道、腦組織類器官,以及糖尿病的研究上更進一步。

流動培養(yǎng)實現(xiàn)血腦屏障三種細胞共培養(yǎng) | 阿爾茨海默病新模型

血腦屏障(blood-brain barrier, BBB)在中樞神經(jīng)系統(tǒng)(CNS)的生理和病理中都起著重要的作用。血腦屏障功能異常會引起包括阿爾茨海默癥(AD)等許多神經(jīng)退行性疾病。組成血腦屏障的毛細血管內(nèi)皮細胞(capillary endothelial cells)、周細胞(pericytes)以及星形膠質(zhì)細胞(astrocytes)間的復雜的相互作用使得很難在體內(nèi)確定這三種細胞對神經(jīng)毒性各自的貢獻。

而Quasi Vivo流動培養(yǎng)系統(tǒng)可為體外培養(yǎng)這三種細胞提供在不形成屏障的情況下維持細胞間通訊的培養(yǎng)環(huán)境。Quasi Vivo流動培養(yǎng)系統(tǒng)為未來研究不同類型的血腦屏障細胞在中樞神經(jīng)系統(tǒng)疾病和細胞毒性試驗中的te殊作用提供一個有價值的工具。(Miranda-Azpiazu P, et al. A novel dynamic multicellular co-culture system for studying individual blood-brain barrier cell types in brain diseases and cytotoxicity testing. Sci Rep. 2018; 8(1): 1-10.)

圖 1. 單du培養(yǎng)的人星形膠質(zhì)細胞(A,GFAP陽性)、周細胞(B,α-actin陽性)、血管內(nèi)皮細胞(C,CD31陽性)以及血管內(nèi)皮細胞形成的緊密連接(D,ZO1陽性)。

圖 2 用Quasi-Vivo QV500培養(yǎng)共享相同的培養(yǎng)基的星形膠質(zhì)細胞、周細胞和血管內(nèi)皮細胞的示意圖(A),R為儲液瓶,P為蠕動泵。連接培養(yǎng)基存儲瓶的一個Quasi-Vivo QV500流動培養(yǎng)系統(tǒng)的細胞培養(yǎng)腔室(B)。

圖 3 Quasi-Vivo QV500流動培養(yǎng)系統(tǒng)建立的能同時培養(yǎng)三種不同細胞的多細胞共培養(yǎng)體系。

圖4 幾種流動培養(yǎng)方式示意圖:A圖為單du星形角質(zhì)細胞流動培養(yǎng),B圖為單du周細胞流動培養(yǎng),C圖為單du血管內(nèi)皮細胞流動培養(yǎng),D圖為三種細胞組合后一起流動培養(yǎng)。

圖5 用MTT法測細胞活力,與靜態(tài)培養(yǎng)相比,采用Quasi-Vivo QV500流動培養(yǎng)系統(tǒng)對單du培養(yǎng)血管內(nèi)皮細胞(HBECs)、周細胞(HBVPs)、星形角質(zhì)細胞(HAs)(A)或三種細胞共培養(yǎng)(B)的血管內(nèi)皮細胞的細胞活力有明顯升高。

圖6 用MTT法測細胞活力,與靜態(tài)培養(yǎng)(Static)相比,流動培養(yǎng)(Dynamic)的周細胞(HBVPs)會更早受到Aβ25-35(淀粉樣蛋白β肽的Aβ25-35片段,用于阿爾茨海默病的造模)的毒害。

總結(jié):本文中研究者利用Quasi-Vivo QV500流動培養(yǎng)系統(tǒng)建立了三種細胞的共培養(yǎng)。這些細胞不接觸,通過共享培養(yǎng)基實現(xiàn)細胞間的通信,不形成屏障能更好的研究這些細胞類型單du對不同化合物的響應情況。并且研究者還發(fā)現(xiàn)共享相同培養(yǎng)基的星形膠質(zhì)細胞、周細胞和血管內(nèi)皮細胞的zui適流速為50 μl/min。

作為創(chuàng)新的細胞培養(yǎng)方法,Quasi Vivo流動培養(yǎng)已經(jīng)quan球70余家專業(yè)機構(gòu)使用驗證,獲得了令人側(cè)目的培養(yǎng)效果,在美、英、法、日等多國開展了頗具新意的細胞研究,涉及呼吸系統(tǒng)、肝臟、腎臟、心血管、成纖維細胞、糖尿病模型、腦組織類器官等。

三、瑞士Ucup3D細胞組織灌注培養(yǎng)生物反應器系統(tǒng)

簡單介紹

UCUP灌注生物反應器是一種用戶友好的工具,用于建立和控制您的3D細胞和組織培養(yǎng)。 Ucup專門設計用于在生命科學相關(guān)領域工作的任何科學家或?qū)嶒炇壹夹g(shù)人員使用,而不必要求使用生物反應器的任何先前經(jīng)驗。應用器官型模型(骨重建,腫瘤微環(huán)境) 3D細胞擴增和分化細胞 - 支架相互作用的研究細胞外基質(zhì)相互作用的研究生成適合臨床前實驗的3D細胞支架結(jié)構(gòu)

產(chǎn)品描述

Ucup三維灌流培養(yǎng)系統(tǒng)

Ucup灌注生物反應器是一種用戶友好的工具,用于建立和控制您的3D細胞和組織培養(yǎng)。

Ucup專門設計用于在生命科學相關(guān)領域工作的任何科學家或?qū)嶒炇壹夹g(shù)人員使用,而不必要求使用生物反應器的任何先前經(jīng)驗。

應用:

器官型模型(骨重建,腫瘤微環(huán)境)

3D細胞擴增和分化

細胞 - 支架相互作用的研究

細胞外基質(zhì)相互作用的研究

生成適合臨床前實驗的3D細胞支架結(jié)構(gòu)

瑞士,Ucup三維灌流培養(yǎng)系統(tǒng)

The Ucup perfusion bioreactor is a user-friendly tool to establish and control your 3D cell and tissue cultures.

Ucup has been specifically designed to be used by any scientist or lab technician working in the life science related field, without necessarily requiring any previous experience with the use of bioreactors. Applications
Organotypic models (Bone remodeling, Tumor microenvironment)
3D cell expansion and differentiation
Investigation of cell-scaffold interactions
Investigation of cell-extracellular matrix interactions
Generation of 3D cell-scaffold constructs suitable for preclinical experimentation
Apply instantly your current cell culture concepts and simply let Ucup further extend them by performing the seamless transition to the 3D context.
Features Advantages Benefits
Direct perfusion Unifrom cell seeding Uniform tissue
Efficient nutrition and waste removal Viable tissue, up to several weeks of culture
Physiological conditions (mimicking inetrstitial fluid flow and associated induced shears Physiologically relevant tissue
Simple and smart design (patented) Easy and ready to use No previous experience with 3D cell cultures required
Minimized manual operations Highly reproducible results
Efficient with many cell types Versatile cell and tissue culture models
Supple scaffold adaptors Compatible with a wide spectrum of 3D porous scaffolds of various composition, architecture and sizes
Access to cell culture medium through valves Suitable to seed and co-culture several cell types, even at different culture time points Possibility to investigate complex cell-cell and cell-extracellular matrix interaction
Efficient cell retrieval from scaffolds after culture (with standard enzymatic treatment) Easy cell analyses (cytofluorimetry, gene expression etc.)
Product Configuration
1x syringe pump
1x rack
10x Ucup disposable bioreactor kits
1x Starter kit
+
+
=
The driving force of the system. It generates the oscillating fluid flow of the cell/medium suspension. It cannot be purchased separately. A rotating rack for easy and correct positioning of Ucup disposable bioreactors. It can also be purchased separately. The central core of the system. It is disposable and it comes with 10x adaptors to fit the specific size of your scaffolds. Scaffolds can also be purchased separately. It provides all what you need to start your 3D cell cultures. Additional accessories (e.g. forceps, syringes) and testing units are also included.
The performance of Ucup has been extensively validated and certified by scientific publications in peer-review journals.
If you are convinced of the benefits that a 3D culture environment can provide,the Ucup bioreactor is the essential tool to conduct with your experiments.
For assistance and advice to set up your experiment, do not hesitate to contact CELLEC’s expert team to address your questions.
應用文獻:
Boccardo and Gaudiello 2016 In this paper, the perfusion-based bioreactor is used for the generation of an adipose mesenchymal stromal cells -based engineered constructs (Title: Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis, Acta Biomaterials)
Cerino 2016 presents an application for engineering an in vitro 3D multi-cellular muscle-like tissue model (Title: Three-dimensional multi-cellular muscle-like tissue engineering in perfusion-based bioreactors, Biotechnology and Bioengineering)
Hirt and Papadimitropoulos 2015 demonstrates the importance of perfusion flow in 3D cultures of tumor cells to efficiently mimic functional features observed “in vivo" and to test anticancer compounds (Title: Bioreactor-engineered cancer tissue-like structures mimic phenotypes, gene expression profiles and drug resistance patterns observed in vivo,Biomaterials)
Centola 2015 In this study, the perfusion-based bioreactor system is used to improve cartilage digestion, resulting in higher and more reproducible yield of cell populations with high proliferation and chondrogenic capacity (Title: An improved cartilage digestion method for research and clinical applications, Tissue Engineering Part C, Methods)
Bao 2015 presents a humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket (Title: Establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system, Virulence)
Papadimitropoulos 2014 presents an efficient expansion method of mesenchymal stromal cells by direct seeding and culturing fresh bone marrow preparation within the pores of 3D porous scaffold (Title: Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion, PLoS One)
Hirt 2014 highlights the potential of perfusion-based models to create 3D tumour microenvironment for cancer immunobiology studies and pre-clinical assessment of innovative treatments (Title: In vitro 3D models of tumor-immune system interaction, Advance Drug Delivery Review)
Papadimitropoulos 2013 presents an application/method for seeding open porous rapid prototyped polymeric scaffolds (Title: A collagen network phase improves cell seeding of open-pore structure scaffolds under perfusion, Journal of Tissue Engineering and Regenerative Medicine)
Sadr 2012 presents an application/method to generate a decellularized cell-laid extacellular matrix which enhances the biological performance of polymeric materials (Title: Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix, Biomaterials)
Gueven 2011 presents an application for upscaling osteogenic and vasculogenic grafts (Title: Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue, Biomaterials)
Papadimitropoulos 2011 presents an application for engineering an in vitro bone organ model (Title: A 3D in vitro bone organ model using human progenitor cells, European Cell & Materials)
Di Maggio 2011 a review for our approaches to engineering in 3D vitro niches (Title: Toward modeling the bone marrow niche using scaffold-based 3D culture systems, Biomaterials)
Santoro 2010 presents an application for upscaling cartilaginous grafts (Title: Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing, Biomaterials)
Scherberich 2007 presents an application for generating osteogenic and vasculogenic grafts (Title: Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity, Stem Cells)
Wendt 2006 describes the system for maintaining living uniform tissues in the scaffolds (Title: Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions, Biorheology)
Braccini 2005 presents an application for generating osteogenic grafts (Title: Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts, Stem Cells)
Wendt 2003 describes the principle of the Ucup and its impact on cell seeding (Title: Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity, Biotechnology and Bioengineering)

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一、意大利SKE品牌意大利多通道三維細胞組織灌注流培養(yǎng)系統(tǒng)

Quasi Vivo細胞灌流培養(yǎng)系統(tǒng)

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Features	Advantages	Benefits
Direct perfusion	Unifrom cell seeding	Uniform tissue
	Efficient nutrition and waste removal	Viable tissue, up to several weeks of culture
	Physiological conditions (mimicking inetrstitial fluid flow and associated induced shears	Physiologically relevant tissue
Simple and smart design (patented)	Easy and ready to use	No previous experience with 3D cell cultures required
	Minimized manual operations	Highly reproducible results
	Efficient with many cell types	Versatile cell and tissue culture models
Supple scaffold adaptors	Compatible with a wide spectrum of 3D porous scaffolds of various composition, architecture and sizes
Access to cell culture medium through valves	Suitable to seed and co-culture several cell types, even at different culture time points	Possibility to investigate complex cell-cell and cell-extracellular matrix interaction
Access to cell culture medium through valves	Efficient cell retrieval from scaffolds after culture (with standard enzymatic treatment)	Easy cell analyses (cytofluorimetry, gene expression etc.)

1x syringe pump		1x rack		10x Ucup disposable bioreactor kits		1x Starter kit
	+		+		=
The driving force of the system. It generates the oscillating fluid flow of the cell/medium suspension. It cannot be purchased separately.		A rotating rack for easy and correct positioning of Ucup disposable bioreactors. It can also be purchased separately.		The central core of the system. It is disposable and it comes with 10x adaptors to fit the specific size of your scaffolds. Scaffolds can also be purchased separately.		It provides all what you need to start your 3D cell cultures. Additional accessories (e.g. forceps, syringes) and testing units are also included.

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一、意大利SKE品牌意大利多通道三維細胞組織灌注流培養(yǎng)系統(tǒng)

Quasi Vivo細胞灌流培養(yǎng)系統(tǒng)

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一、意大利SKE品牌意大利多通道三維細胞組織灌注流培養(yǎng)系統(tǒng)