b16f10細(xì)胞-小鼠皮膚黑色素瘤細(xì)胞

b16f10細(xì)胞是B16-F0的亞系.

細(xì)胞名稱： 小鼠皮膚黑色素瘤細(xì)胞

簡(jiǎn)稱：b16f10細(xì)胞

細(xì)胞別名： B16/F10; B16 F10; B16F10; B16 melanoma F10

貨號(hào)：TCM-C713

規(guī)格： 1X10^6

價(jià)格：140 0元 (干冰運(yùn)輸需另加200元干冰費(fèi))  

種屬來(lái)源：小鼠

組織來(lái)源：皮膚

特征：黑色素瘤

細(xì)胞形態(tài)：上皮細(xì)胞樣混有梭形細(xì)胞

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：DMEM-H+10%FBS+1%P/S

b16f10細(xì)胞培養(yǎng)基貨號(hào)：TCM-G713

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

b16f10細(xì)胞凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790

Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987

Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034

Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927

Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183

Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654

Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469