u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞
細(xì)胞名稱:u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞(STR鑒定正確)
細(xì)胞別稱: U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG
細(xì)胞簡(jiǎn)稱:u251細(xì)胞株
產(chǎn)品貨號(hào):TCH-C366
規(guī)格: 1×10?cells/ T25 培養(yǎng)瓶
價(jià)格:1000元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來(lái)源:人
組織來(lái)源:神經(jīng)膠質(zhì)
特征:神經(jīng)膠質(zhì)細(xì)胞瘤
細(xì)胞形態(tài):成纖維細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系:DMEM-H+10%FBS+1%P/S
u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞 配套培養(yǎng)基貨號(hào): TCH-G366
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞 分離于膠質(zhì)母細(xì)胞瘤組織。
參考文獻(xiàn):
"Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)
Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.
Origin of the U87MG glioma cell line: good news and bad news.
Sci. Transl. Med. 8:354re3-354re3(2016)
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)"
-
- 尊敬的訪客:
當(dāng)您來(lái)到這個(gè)頁(yè)面時(shí),您已成為我們最尊貴的客戶,蘇州阿爾法生物實(shí)驗(yàn)器材有限公司真誠(chéng)歡迎您的到來(lái)!
-
- 聯(lián)系我們免費(fèi)服務(wù)電話:十三年專(zhuān)注于生物實(shí)驗(yàn)室儀器供應(yīng)